Investigations on Microbes Attached to the Cuticle of Phytonematodes

Affiliation
Laboratory of Insect and Nematode Management, Corteva Agriscience Research, Center Eschbach, Eschbach, Germany
Topalović, Olivera;
Affiliation
Laboratory of Insect and Nematode Management, Corteva Agriscience Research, Center Eschbach, Eschbach, Germany
Radaković, Zoran S.;
GND
1139127454
Affiliation
Julius Kühn Institute (JKI), Institute for Epidemiology and Pathogen Diagnostics, Germany
Elhady, Ahmed;
Affiliation
Rheinische Friedrich-Wilhelms-University of Bonn, INRES—Molecular Phytomedicine, Bonn, Germany
Bredenbruch, Sandra;
GND
1058940058
Affiliation
Julius Kühn Institute (JKI), Institute for Epidemiology and Pathogen Diagnostics, Germany
Heuer, Holger

Nematodes form various associations with soil microbiome. Experimental studies on nematode-attached microbes can improve mechanistic understanding of these associations and lead to new discoveries relevant for the field of nematode biocontrol. Microbial attachment to the surface of phytonematodes is very specific and influenced by a multitude of factors, including the designation of nematodes and microbes, environmental and biological factors in soil, time of incubation, and the ratio and evolutionary trajectories between nematodes and microbes. Here, we describe how the classical nematological and microbiological techniques can be coupled with the advanced molecular tools to study the microbial attachment to phytonematodes in soil. We focus on the characterization of nematode-attached microbes using classical microbiological approaches and high-throughput amplicon sequencing and on the effects of nematode-attached microbes on plant defense responses.

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