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Grapevine DNA polymorphisms revealed by microsatellite-derived markers from soybean and rice

Zugehörigkeit
CSIR National Chemical Laboratory, Pune, India
Agrawal, D. C.;
GND
1059151928
Zugehörigkeit
Federal Centre for Breeding Research on Cultivated Plants, Institute for Grapevine Breeding Geilweilerhof, Siebeldingen, Germany
Töpfer, Reinhard;
GND
1059152029
Zugehörigkeit
Federal Centre for Breeding Research on Cultivated Plants, Institute for Grapevine Breeding Geilweilerhof, Siebeldingen, Germany
Zyprian, Eva

We report detection of DNA polymorphisms in grapevine by the use of microsatellite-flanking primer pairs from soybean and rice. These “cross species” microsatellite-derived markers were checked for their inheritance patterns in controlled grapevine crosses. They produced multiple bands that segregated and can be scored as individual genetic markers of dominant type. Employed in genetic mapping studies they offer advantages such as improved reproducibility in comparison to commonly used multi-locus marker systems like RAPDs and AFLPs.

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