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Comparison of high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and ultra-high performance liquid chromatography with evaporative light scattering (UHPLC-ELSD) for the analyses of fructooligosaccharides in onion (Allium cepa L.)

Affiliation
University of Hohenheim, Institute of Food Science and Biotechnology, Chair of Plant Foodstuff Technology and Analysis, Garbenstrasse 25, D-70599 Stuttgart, Germany
Pöhnl, Tobias;
GND
129256323
Affiliation
Julius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Ecological Chemistry, Plant Analysis and Stored Product Protection, Berlin, Germany
Böttcher, Christoph;
GND
140592741
Affiliation
Julius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Ecological Chemistry, Plant Analysis and Stored Product Protection, Berlin, Germany
Schulz, Hartwig;
Affiliation
Symrise AG, Mühlenfeldstrasse 1, D-37603 Holzminden, Germany
Stürtz, Melanie;
Affiliation
Symrise AG, Mühlenfeldstrasse 1, D-37603 Holzminden, Germany
Widder, Sabine;
Affiliation
University of Hohenheim, Institute of Food Science and Biotechnology, Chair of Plant Foodstuff Technology and Analysis, Garbenstrasse 25, D-70599 Stuttgart, Germany ; King Abdulaziz University, Faculty of Science, Biological Science Department, P.O. Box 80257, Jeddah 21589, Saudi Arabia
Carle, Reinhold;
Affiliation
University of Hohenheim, Institute of Food Science and Biotechnology, Chair of Plant Foodstuff Technology and Analysis, Garbenstrasse 25, D-70599 Stuttgart, Germany
Schweiggert, Ralf M.

Fructooligosaccharides (FOS) in foods are controversially discussed due to their probiotic activity and their putative contribution to the irritable bowel syndrome (IBS). Besides their nutritional relevance, the genuine composition of carbohydrates in onions has high impact on non-enzymatic browning during thermal processing of onions. In the present study, a method based on high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) was validated and compared to an ultra-high performance liquid chromatographic method using evaporative light scattering detection (UHPLC-ELSD) for analysing saccharide contents of various onion (Allium cepa L.) cultivars substantially differing in their FOS pattern. Analyte identity was confirmed by authentic standards and UHPLC-electrospray ionization-high resolution mass spectrometry (UHPLC-ESI-HRMS). A total of 41 saccharides was detected with both methods, including FOS with a DP of up to 20. Specific validation parameters obtained with authentic standards are presented for FOS with a DP of up to 9. Although run times were significantly shorter using the UHPLC method, baseline separation and analytical range of FOS with higher DP were clearly superior using HPAEC-PAD, reaching lower quantification limits (0.12–2.3 mg/L) compared to UHPLC-ELSD (34–68 mg/L). This work provides guidance for method selection when analysing FOS from plant samples.

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