Vergleich serologischer Tests zur Diagnostik der enzootischen Rinderleukose im Rahmen der Sanierung eines Großbestandes

The anti-BLV-EIA (Dessau) and immuno-diffusion test were used to detect and eradicate BLV infection in a large dairy herd comprising 2000 animals. The testing of 79 cattle which reacted positively once or more to the EIA revealed that the additional use of the IDT was not advantageous. Other serological tests, with a greater sensitivity than the IDT or the anti-BLV-EIA were used with four selected trial groups of cattle. An inhibition test and anti-BLV-EIA based on monoclonal antibody to detect the envelope protein gp51 were used. An additional experiment in sheep was performed for the direct detection of BLV-specific protein from short time-cultured lymphocytes. The results of these comparisons are described and discussed