A new putative G-protein coupled receptor gene associated with the immune system of rainbow trout (Oncorhynchus mykiss)

A putative G-protein coupled receptor (GPCR) gene belonging to the rhodopsin-like family was detected in rainbow trout and designated LPSenhR-1. Only moderate homology (<35%) was present with known GPCRs. Semi-quantitative RT-PCR indicated that the gene was expressed predominantly in lymphoid tissues, with highest expression associated with cells of the monocyte/macrophage lineage. Expression was strongly up-regulated by injection with LPS but not by infection with infectious haematopoietic necrosis virus.