Quantification of Wheat spindle streak mosaic virus and Soil borne cereal mosaic virus in resistance testing of field samples of triticale using real-time RT-PCR

Triticale, a cross between rye and wheat, is a crop important for animal feed and the production of biogas and ethanol. Soil-borne viruses found in wheat and rye, such as Furo­viruses and Bymoviruses, also infect triticale. In order to evaluate resistance/tolerance it is necessary to accuratelyquantify virus content in the plants. We have developedRT-qPCR assays for the quantitative detection of the Bymovirus Wheat spindle streak mosaic Virus (WSSMV) and the Furovirus Soil-borne cereal mosaic virus (SBCMV) in fieldsamples. Both a SYBR Green and a hydrolysis probe approachwere tested. The RT-qPCR approach allows the quantitativeevaluation and differentiation of triticale resistance toWSSMV and SBCMV. The reproducible large-scale analysis of field samples is feasible.