High-throughput screening for protein content in blue, yellow and white lupins

Different lupin species (blue lupins, L. angustifolius; yellow lupins, L. luteus; white lupins, L. albus) were analyzed for the protein content in the growing seasons 2010 and 2012. As a reference method for the determination of the protein content, the Kjeldahl method was used. Based on these data a high-throughput screening method for whole lupin seeds was developed using NIRS analyses on a Bruker Multi Purpose Analyzer. The best calibration, cross validation, and prediction of independent samples was observed for whole seeds of blue lupins (RMSECV=0.924, R²=0.82) followed by yellow (RMSECV=1.05, R²=0.67) and white lupin seeds (RMSECV=1.08, R²=0.65).