Pine Wilt Disease Conference 2013 : 15th to 18th Oct. 2013, Braunschweig / Germany ; Scientific Conference IUFRO unit 7.02.10 and FP7 EU-Research Project REPHRAME - Abstracts

Content

• Präambel

  p. 1

  Schröder, Thomas

• Sampling strategies for Monochamus sutor – a potential vector of the pine wood nematode

  p. 12

  Schroeder, Martin

• Assessing potential expansion of the Pine Wood Nematode (Bursaphelenchus xylophilus) from the spatial genetic structure of the vector (Monochamus galloprovincialis)

  p. 13

  Haran, Julien; Roques, Alain; Roux, Géraldine

• Effective traps for live trapping of PWN vectors Monochamus spp.

  p. 14

  Alvarez, Gonzalo; Etxebeste, Iñaki; Sánchez, Estela; Gallego, Diego; Pajares, Juan A.

• Effective attraction radius of pheromone traps for Monochamus galloprovincialis

  p. 17

  Jactel, Hervé

• Monochamus galloprovincialis dispersal and effective sampling area of operational traps

  p. 18

  Etxebeste, Iñaki; Álvarez, Gonzalo; Sánchez-Husillos, Estela; Pajares, Juan A.

• Flight performances of Monochamus galloprovincialis, insect vector of the Pine Wood nematode

  p. 20

  David, Guillaume; Jactel, Hervé; Piou, Dominique; Naves, Pedro; Sousa, Edmundo de

• Simulating the dispersal of Monochamus galloprovinciallis based on its flight mill performance and testing several management scenarios

  p. 21

  Robinet, Christelle; David, Guillaume; Piou, Dominique; Roques, Alain; Jactel, Hervé

• Dispersal behavior and long distance flight capacity of Monochamus galloprovincialis (Olivier 1795)

  p. 22

  Mas, H.; Hernández, R.; Villaroya, M.; Sánchez, G.; Pérez-Laorga, E.; González, E.; Ortiz, A.; Lencina, J. L.; Rovira, J.; Marco, M.; Pérez, V.; Gil, M.; Sánchez-García, F. J.; Bordón, P.; Pastor, C.; Biel, M. J.; Montagud, L.; Gallego, D.

• Smart-traps combined with molecular on-site detection to monitor Monochamus spp. and associated pine wood nematode

  p. 23

  Chinellato, Fabio; Simonato, Mauro; Battisti, Andrea; Faccoli, Massimo; Hardwick, Scott; Suckling, D. Max

• Physiological development of Monochamus galloprovincialis immature adults through shoot feeding

  p. 26

  Sánchez-Husillos, Estela; Etxebeste, Iñaki; Álvarez-Baz, G.; Pajares, Juan Alonso

• Detection of exoctic Monochamus spp. in Belgium – testing the tools in the area of origin

  p. 28

  Boone Celia K.; Grégoire Jean-C.; Berkvens Nick; Casteels Hans; Viaene Nicole

• Preliminary results of potential vector species of Bursaphelenchus spp. (Nematoda:Parasitaphelenchidae) in Turkey

  p. 29

  Dayi, Mehmet; Akbulut, Süleyman

• Validation of a real time PCR assay for the detection of Bursaphelenchus xylophilus in targeted matrices in the framework of national survey

  p. 31

  Anthoine, Geraldine; Chappé, Anne-Marie

• Molecular detection of B. xylophilus in complex DNA backgrounds

  p. 33

  Landeweert, Renske; Mooijman, Paul; Mulder, Winfried; Elsen, Sven van den; Helder, Johannes

• Pinewood nematode assay and development of real-time PCR for species identification in North Carolina Department of Agriculture & Consumer Services

  p. 35

  Ye, Weimin; Giblin-Davis, Robin M.

• Development of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method to detect living pinewood nematode, Bursaphelenchus xylophilus, in wood.

  p. 39

  Leal, Isabel; Allen, Eric; Anema, Jennifer; Reisle, C.; Foord, Brett; Uzunovic, Adnan; Varga, Aniko; James, Delano

• EUPHRESCO BURSA Project: early detection methods of the pinewood nematode

  p. 41

  Anthoine, Geraldine

• Occurrence and distribution of Bursaphelenchus species in Switzerland

  p. 43

  Prospero, Simone; Polomski, Janina; Rigling, Daniel

• Coniferous bark hot steam treatment for the elimination of the pinewood nematode

  p. 44

  Fonseca, Luis; Santos, José; Nestler, Hartmut; Verdasca, Joaquim; Oliveira, Rui; Abrantes, Isabel; Serra, Clara

• Non vector spread of Bursaphelenchus xylophilus via wood chips

  p. 46

  Hopf, Andrea; Schröder, Thomas

• Complementary studies to validate the proposed fumigation schedules of sulfuryl fluoride for inclusion in ISPM No. 15 for the eradication of Bursaphelenchus xylophilus from wood packaging material

  p. 48

  Bonifácio, L.; Inácio, M. L.; Sousa, E.; Buckley, S.; Thoms, E. M.

• Efficacy of kiln drying as phytosanitary treatment against wood borne nematodes

  p. 51

  Schröder, Thomas; Welling, Johannes; Aukamp-Timmreck, Claudia

• Recent research on pine wilt disease in Russia

  p. 54

  Kulinich, O. A.; Arbuzova, E. N.; Magomedov, U. Sh.; Kozyreva, N. I.; Mazurin, E. S.; Kolychikhina, M. S.; Ryss, A. Yu

• Inoculation effects of Pinus thunbergii with Bursaphelenchus xylophilus and two strains of Bacillus firmus

  p. 56

  Tan, Jiajin; Qu, Hongye; Hao, Dejun; Chen, Fengmao

• Bursaphelenchus xylophilus and associated bacteria under oxidative stress conditions

  p. 58

  Vicente, Cláudia S. L.; Ikuyo, Yoriko; Mota, Manuel; Hasegawa, Koichi

• Diversity and in vitro nematicidal activity of bacteria associated to pinewood nematode

  p. 60

  Morais, Paula V.; Proença, Diogo Neves; Paiva, Gabriel; Francisco, Romeu; Verissimo, Paula; Fonseca, Luís; Abrantes, Isabel M. O.

• Natural bacterial communities associated with the pine sawyer beetle Monochamus galloprovincialis

  p. 63

  Vicente, Cláudia S. L.; Nascimento, F. X.; Espada, Margarida; Barbosa, Pedro; Hasegawa, Koichi; Mota, Manuel; Oliveira, Solange

• A research project to develop strategic action plan in the pine-wilt-disease unaffected area in Northern Japan

  p. 66

  Nakamura, Katsunori; Maehara, Noritoshi; Aikawa, Takuya; Ichihara, Yu; Kosaka, Hajime; Kanzaki, N.; Kagaya, Etsuko; Sugita, Hisahsi; Masaki, Takashi; Kimura, Koki; Kon, Jun-ichi; Kaneko, Tomonori

• Study on the techniques of sustainable control of pine wood nematode disease (Bursaphelenchus xylophilus)

  p. 67

  Xu, Fuyuan; Xu, Ming; Zheng, Huaying; Xie, Chunxia; Liu, Yunpeng; Gao, Yue

• New strategies for pine wilt disease (PWD) management in Portugal: preventive methods to reduce the spread of the disease to new areas

  p. 68

  Naves, Pedro; Vieira, M.; Sousa, Edmundo de

• Inter-specific competiton of Bursaphelenchus xylophilus with native populations of B. mucronatus in pine

  p. 70

  Tomalak, Marek; Filipiak. Anna

• Interspecific hybridization between Bursaphelenchus xylophilus and Bursaphelenchus mucronatus

  p. 72

  Zhengmin, Han; Ye, Guo; Ailing, Ben; Cao, Dongxia

• Phenotypic and genotypic traits of recombinant inbred lines of pine wood nematode, Bursaphelenchus xylophilus

  p. 74

  Kato, Tetsuro; Kaneko, Akira; Shinya, Ryoji; Futai, Kazuyoshi; Takeuchi, Yuko

• Pathogenicity, reproduction and survival of axenic Bursaphelenchus xylophilus

  p. 79

  Zhu, Lihua; Ye, Jianren; Huang, Lin

• Influence of pine wood nematode invasion on typical Masson pine ecosystem in Three Gorges Reservoir Region of China

  p. 80

  Gao, Rui-he; Shi, Juan; Luo, You-qing

• Using an evapo-transpiration model to predict the current and future range and severity of pine wilt disease caused by pine wood nematode, Bursaphelenchus xylophilus in Europe

  p. 82

  Gruffudd, Hannah; Evans, Hugh F.; Jenkins, Tom

• Cold-tolerance and adaption of pine wood nematode in China

  p. 84

  Huang, Rui-fen; Shi, Juan; Luo, You-qing

• A comparative proteomics analysis on resistant provenance of Pinus massoniana inoculated with Bursaphelenchus xylophilus

  p. 86

  Zheng, Huaying; Xu, Ming; Xu, Fuyuan

• Comparative transcriptomics to understand the molecular basis of Bursaphelenchus xylophilus pathogenicity

  p. 87

  Egas, Conceição; Leisico, Francisco; Duarte, T.; Gomes, Paula; Fonseca, Luís; Abrantes, Isabel; Pinheiro, Miguel

• The Function of Major Sperm Proteins (MSPs) in reproduction of pine wood nematode

  p. 90

  Huang, Lin; Tian, Minqi; Qiu, Xiuwen; Zhang, Yi; Wu, Xiaoqin; Ye, Jianren

• Exploring the relation between virulence and oxidative stress response of Bursaphelenchus xylophilus and Bursaphelenchus mucronatus

  p. 93

  Vicente, Claudia S. L.; Ikuyo, Yoriko; Mota, Manuel; Hasegawa, Koichi

• Bursaphelenchus xylophilus identification, from literature to routine analysis: how to make morphological analysis reliable

  p. 96

  Sarniguet, C.; Buisson, A.; Anthoine, Geraldine

• Genetic diversity of the pinewood nematode, after 100 years of invasion in Japan

  p. 98

  Akiba, M.; Kanzaki. N.; Sahashi, N.

• Bursaphelenchus mucronatus as a cause of dying a secondary fir forests in Ukrainian Carpathians

  p. 100

  Kozlovsky, Mykola

• Growth properties of pine trees died from pine wilt disease

  p. 101

  Ikeda, Takefumi; Kobayashi, Keisuke; Naoe, Shoji

• Testing attractants for trapping Monochamus sartor and Monochamus sutor

  p. 103

  Hoch, G.; Halbig, P.; Menschhorn, P.; Hall, D. R.; Krehan, H.

• Inhabitation of the pinewood nematode and its vectors in the tsunami-damaged Pinus thunbergii and P. densiflora trees

  p. 105

  Nakamura, K.; Aikawa, T.; Ichihara, Y.; Maehara, N.; Mizuta, N.

• Bacterial flora and its association with the pine wood nematode (Bursaphelenchus xylophilus)

  p. 106

  Kato, T.; Futai, K.; Takeuchi, Y.

• Embolism development observed with a compact MRI in Japanese black pine clones resistant to pine wilt disease

  p. 110

  Akami, A.; Kusumoto, D.; Hirao, T.; Watanabe, A.; Fukuda, K.

• Analysis of genetic diversity of Bursaphelenchus mucronatus and B. xylophilus isolates based on ISSR markers

  p. 112

  Liu, F. Y.; Chen, F. M.; Xie, L.; Ye, J. R.; Tan, J. J.

• Studies on non-vector transmission of Bursaphelenchus xylophilus on Pinus pinaster

  p. 115

  Magnusson, C.; Henriques, J.; Sousa, E.

• Bursaphelenchus xylophilus does not occur in Belgium, but what about its vectors, the Monochamus spp.?

  p. 116

  Berkvens, N.; Casteels, H.; Damme, N.; Bighiu, A.; Witters, J.; Grégoire, J.-C.; Boone, C.; Michelante, D.; Viaene, N.

• Construction of engineering bacterium expressing flagellin of Pseudomonas fluorescens and its toxicity to Pinus thunbergii in vivo

  p. 118

  Nan, Li Sheng; Zhao, Bo Guang; Li, Rong Gui; Guo, Dao Sen

• Gene silencing in Bursaphelenchus xylophilus: knock down of a calponin gene and its effect on nematodes movement

  p. 120

  Cardoso, J. M.; Fonseca, L.; Gomes, P.; Egas, C.; Abrantes, I.

• Comparative analysis of MspI satellite repeats of the pinewood nematode, Bursaphelenchus xylophilus, at different geographic scales

  p. 123

  Vieira, P.; Castagnone, C.; Mallez, S.; Espada, M.; Navas, A.; Mota, M.; Castagnone-Sereno, P.

• Physiological responses to water stress and temperature on the pine wilt disease development in Pinus spp.

  p. 124

  Abrantes, I.; Fonseca, L.; Fernandes, P.; Mendes, A.; Colwell, F.; Costa, C.; Máguas, C.; Correia, O.

• Improved key to the species of the xylophilus group of the genus Bursaphelenchus Fuchs, 1937

  p. 127

  Braasch, Helen; Schönfeld, Ute

• Vacuum pressure impregnation for the elimination of the pinewood nematode from pine wood

  p. 129

  Fonseca, L.; Santos, A.; Amado, S.; Pedroso, M.; Abrantes, I.

• Pine wood nematodes - as a factor of pine decline in Croatia

  p. 131

  Đođ, N.; Pernek, M.; Carletti, B.

• The use of PCR-HRM technique for detection of the quarantine nematode Bursaphelenchus xylophilus

  p. 133

  Filipiak, Anna; Tomalak, Marek

• Modelling of potential spread of Pine Wood Nematode by natural means in Slovenia at present climate conditions and in light of predicted climate changes

  p. 136

  Gerič Stare, B.; Knapič, M.; Ogrica, N.; Širca, S.; Urek, G.

• Analysis of transcriptional expression variation of pine wood nematode (Bursaphelenchus xylophilus) using EST-based simple sequence repeats

  p. 138

  Xu, Jun-Rong; Wu, Xiao-Qin; Fang, Xin; Ye, Jian-Ren; Liu, Yun; Xu, Lu; Lu, Yao

• The Dutch approach to Pine Wilt Disease (PWD): longhorn beetles and nematodes under surveillance

  p. 140

  Bruggen, A. S. van; Nijs, L. J. M. F den; Loomans, A. J. M.