Sample Area Sampling Place Fermentation Duration Fermentation Type LAB 1 LAB 2 Enterobacteria 1 Enterobacteria 2 TAC 1 TAC 2 Yeast 1 Yeast 2 pH 1 pH 2 A01 (Hexanoic acid methyl ester) A03 (Unknown01) A04 (Heptanoic acid methyl ester) A05 (3-Hydroxy-2-butanone (Acetoin)) A06 (Octanoic acid methyl ester) A07 (Acetic acid) A08 (Nonanoic acid methyl ester) A09 (Propanoic acid) A10 (2.3-Butanediol) A12 (Isobutyric acid) A13 (Decanoic acid methyl ester) A16 (Butanoic acid) A18 (Isovaleric acid) A19 (Unknown05) A20 (Pentanoic acid) A22 (Dodecanoic acid methyl ester) A23 (Hexanoic acid) A25 (Heptanoic acid) A26 (Fatty acid methyl ester, probably branched) A27 (Tetradecanoic acid methyl ester) A28 (Octanoic acid) A29 (Methyltetradecanoic acid or isomer) A30 (12-Methyltetradecanoic acid) A31 (Pentadecanoic acid methyl ester) A32 (Hexadecanoic acid methyl ester) A33 (Unknown05) A34 (Unknown06) W1 Wolkite (South-western Ethiopia) Household A 309 Surface fermentation followed by pit fermentation 620 3800 bLOD bLOD 130 840 bLOD bLOD 3.7 3.7 0.053186 0.268878 0.018551 1.668294 0.136932 12.466062 0.049293 3.643876 0.356635 0.196843 0.074233 13.380855 0.115055 0.016151 1.165319 0.076289 0.67413 0.046927 0.0256 0.0405 0.054632 0.02827 0.051496 0.005825 0.081133 0.020965 0.009221 W2 Wolkite (South-western Ethiopia) Household B 309 Surface fermentation followed by pit fermentation NA NA NA NA NA NA NA NA NA NA 0.06349 0.02053 0.059907 0.514611 0.176862 9.878705 0.020968 2.674546 0.225676 0.1742 0.047633 13.953082 0.11495 0.029065 1.129336 0.062055 0.737506 0.05251 0.019792 0.0749 0.074995 0.021542 0.094863 0.010782 0.10527 0.024847 0.013456 W3 Wolkite (South-western Ethiopia) Household A 279 Surface fermentation followed by pit fermentation 1700 700 bLOD bLOD 100 370 bLOD bLOD 4 3.4 0.009075 0.256849 0.007588 0.474503 0.048467 8.999955 0.015736 3.820391 0.271548 0.094648 0.024055 11.88263 0.085212 0.029386 0.500984 0.013543 0.197594 0.04209 0.010135 0.016345 0.065138 0.010872 0.009193 0.0036 0.064664 0.01336 0.004621 W4 Wolkite (South-western Ethiopia) Household A 249 Surface fermentation followed by pit fermentation NA NA NA NA NA NA NA NA NA NA 0.04722 0.169226 0.02658 0.142442 0.146286 9.913439 0.042254 2.714984 0.114886 0.094012 0.063173 9.938846 0.09254 0.020398 1.578596 0.039681 0.503498 0.049892 0.021369 0.030924 0.056956 0.013665 0.034919 0.004622 0.045423 0.022065 0.007031 W5 Wolkite (South-western Ethiopia) Household A 159 Surface fermentation followed by pit fermentation NA NA NA NA NA NA NA NA NA NA 0.034595 0.199028 0.016163 1.153027 0.096732 8.37545 0.017856 2.960428 0.176365 0.15273 0.033459 12.992117 0.15557 0.022513 2.334132 0.036886 1.113508 0.103053 0.010042 0.018687 0.134878 0.021352 0.017754 0.002737 0.036357 0.012747 0.013664 W6 Wolkite (South-western Ethiopia) Household B 249 Surface fermentation followed by pit fermentation 120 bLOD bLOD bLOD 1400 1700 bLOD bLOD 3.4 3.6 0.101052 0.267963 0.026145 3.538991 0.147811 3.780315 0.029633 1.186654 0.032184 0.132058 0.02944 12.279385 0.113635 0.015134 3.366397 0.014334 2.855707 0.141808 0.010687 0.028755 0.177113 NA 0.017964 0.00335 0.065196 0.023043 0.006752 W7 Wolkite (South-western Ethiopia) Household B 219 Surface fermentation followed by pit fermentation NA NA NA NA NA NA NA NA NA NA 0.386367 0.292312 0.079981 2.631397 0.304242 4.729692 0.120049 1.389982 0.212721 0.125134 0.246812 14.560353 0.124549 0.022929 2.959106 0.13527 2.95492 0.081448 0.063754 0.069716 0.062295 0.069403 0.307401 0.015465 0.189405 0.050453 0.013538 W8 Wolkite (South-western Ethiopia) Household A 30 Surface fermentation followed by pit fermentation 220000000 340000000 4600 10000 44000 58000 15000 17000 4.6 5 0.009047 0.421898 0.011407 0.362509 0.139577 5.849786 0.103008 0.862645 0.027823 0.030798 0.156627 9.924006 0.026832 0.01785 0.083869 0.202583 0.08295 0.018267 0.171094 0.738103 0.036824 0.07683 0.215555 0.073676 0.571142 0.262481 0.019191 W9 Wolkite (South-western Ethiopia) Household A 20 Surface fermentation followed by pit fermentation 1800000000 850000000 3500 4400 25000 41000 1600 2600 4.8 4.9 0.002563 0.330175 0.001944 0.142812 0.020559 5.123798 0.054788 0.509898 0.062488 0.024126 0.102398 18.013639 0.022254 0.009781 0.098529 0.045781 0.074537 0.006295 0.037888 0.05856 0.021183 0.016251 0.051466 0.012322 0.202013 0.028779 0.014891 W10 Wolkite (South-western Ethiopia) Household A 10 Surface fermentation followed by pit fermentation 15000000 42000000 140000 500000 6300 58000 130 120 4.9 5.4 0.004243 0.046042 0.003475 0.021367 0.030457 4.645329 0.023628 0.475864 0.074728 0.035055 0.036546 11.95717 0.013348 0.022223 0.084616 0.035074 0.073238 0.006623 0.02357 0.087974 0.015411 0.014828 0.025989 0.010725 0.077522 0.03987 0.005797 D1 Dilla (South Ethiopia) Household C 7 Surface fermentation with immediate addition of starter cultures (backslopping) 240000000 440000000 160 230 35000 80000 130 200 4.9 4.8 0.005583 0.435123 0.002961 0.136716 0.05311 2.156077 0.195442 0.119452 0.046981 0.026262 0.31596 1.347034 0.0176 0.013327 0.023773 0.11306 0.023945 NA 0.012094 0.088286 0.004043 0.008859 0.023893 0.052532 1.43195 0.017617 0.021074 D2 Dilla (South Ethiopia) Household C 14 Surface fermentation with immediate addition of starter cultures (backslopping) 150000000 280000000 13 bLOD 7400 32000 1000 2500 4.1 4.3 0.001972 0.048019 NA 0.104283 0.003872 2.015333 NA 0.19174 0.070037 0.026553 0.009261 1.955073 0.017064 0.011925 0.046726 0.006238 0.045763 NA NA 0.003623 0.003281 NA 0.002321 NA 0.044793 NA NA D3 Dilla (South Ethiopia) Local market approximately 14 Surface fermentation with immediate addition of starter cultures (backslopping) 1900000 3000000 22 29 73000 86000 120 810 4.4 4.7 0.020189 0.277037 0.008697 0.024398 0.120155 2.265676 0.393076 0.579807 0.030179 0.029008 3.105198 3.11114 0.047019 NA 0.228339 1.074228 0.026582 NA 0.05446 0.6414 NA 0.078439 0.201587 0.298056 11.321878 0.130904 0.011009 G2 Ginchi (Western Ethiopia) Household D 111 Surface fermentation followed by pit fermentation NA NA NA NA NA NA NA NA NA NA 0.04714 0.29403 0.020095 0.268448 0.081461 3.906191 0.124616 0.770418 0.033708 0.10764 0.210138 16.289412 0.05945 0.007304 0.288885 0.122102 0.557685 0.021484 0.066795 0.065314 0.01852 0.029971 0.216138 0.03282 0.42524 0.029108 0.006044 G3 Ginchi (Western Ethiopia) Household D 28 Surface fermentation followed by pit fermentation NA NA NA NA NA NA NA NA NA NA 0.004483 0.031521 0.003566 0.76996 0.036613 6.465865 0.055756 2.214081 0.219858 0.089956 0.121733 14.463386 0.052696 0.009309 0.155924 0.088149 0.037504 0.004547 0.039149 0.073927 0.01166 0.02209 0.128793 0.042933 0.299177 0.032764 0.011265 Wol-1 Woliso (South-western Ethiopia) Local market approximately 90 Pit fermentation with addition of starter cultures (backslopping) 700000 61000 250 6800 1800 8200 11000 10000 3.8 3.9 0.002586 0.031321 0.002379 0.066577 0.012839 4.582956 0.015365 1.458476 0.02499 0.013194 0.015682 3.791879 0.010644 0.00835 0.082501 0.00607 0.065121 0.00535 NA 0.00623 0.011679 NA 0.00156 NA 0.048379 0.0029 0.01716 Wol-2 Woliso (South-western Ethiopia) Local market approximately 90 Pit fermentation with addition of starter cultures (backslopping) NA NA NA NA NA NA NA NA NA NA 0.00237 0.759955 NA NA 0.0372 3.298483 0.091786 1.83182 0.024756 0.024957 0.033308 3.448992 NA NA 0.049613 0.019113 0.046743 0.005059 NA 0.014543 0.009508 NA NA 0.005431 0.297468 NA NA