The suitability of cytochrome-P4501A1 as a biomarker for PCB contamination in European eel (Anguilla anguilla)
European eel (Anguilla anguilla) elvers were intraperitoneally injected with different doses of 3,3,4,4-trachlorobiyphenyl(PCB77)toexamineandcharacterizetheinductiveeffectofcoplanarPCBsonCYP1A1 gene expression in liver and gills by using a semi-quantitative RT-PCR analysis. The influence of PCB77 injectionontranscriptionactivityofthehousekeepinggeneglyceraldehyde-3-phosphate-dehydrogenase (GAPDH)wastestedtodetermineitssuitabilityasareferencegeneforfurtherquantitativegeneexpression analyses. Our results clearly indicate a significant dose-dependent increase in CYP1A1 gene expression in the gills of European eel, while in liver tissues a significant elevation in CYP1A1 gene expression was only detectableathighestcontaminationrates,indicatingthepotentialofCYP1A1differentialgeneexpression analysis in gills as a biomarker for PCB contamination in eels. PCB77 contamination did not affect GAPDH transcription in gills but, at highest doses, resulted in a significant elevation in liver, speaking against GAPDH as a reference housekeeping gene after PCB exposure.
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