In vitro regeneration of gerbera

A system for in vitro regeneration of adventitious shoots from callus was developed in current work. Callus was formed from in vitro juvenile leaf explants of the Gerbera “Gerbera jamesonii” on 58 different MS-based media containing different concentration and combinations of plant growth regulators, i.e., BAP, Kin, Zeatin, NAA, IAA and ABA. Two different shoot formation media were evaluated, both contain 2 mg/l NAA but they have different concentration of BAP (2.0 and 4 mg/l). The highest shoot regeneration frequency was 36.6 % which was obtained on induction medium containing 2 mg/l BAP and 0.25 mg/l ABA and shoot formation medium containing 2 mg/l of each of BAP and NAA. Regenerated shoots were successfully rooted on MS medium containing 40 µg/l NAA.