Viability of Moringa oleifera seeds stored at different temperatures and recent status of Moringa oleifera collections in seed banks worldwide

Rapid decline in seed viability of Moringa oleifera Lam. due to storage severely reduces its capability to germinate. This study aimed to evaluate the viability of moringa seeds stored at various temperatures through seed germination on a selected medium and provide insights into its conservation in seed banks. The research consisted of two experiments. (1) Optimization of moringa seed
germination using different media: A) sand 100%; B) mixed sand, soil, manure, rice husk charcoal (1:1:1:1); C) mixed sand, soil, and manure (1:1:1); D) mixed soil, manure, rice husk charcoal (2:1:1); and E) soil 100%. (2) Determination of moringa seed viability stored at various temperatures (-35, 5, 20, and 25 °C) every three months for 24 months. The information on the moringa seed banks was compiled via a literature review. Germination variables (percentage, rate, time, and index) were observed and recorded. The best germination medium for moringa seeds was medium B. Storage temperature at 5 °C was the most suitable temperature to maintain viability, indicated by the highest germination percentage, rate, and index, and the shortest germination time of only 6 days. There are moringa seed collections of 11 seed banks published online.