Temperature as Potential Key Regulator for Ebola virus Replication in Primary Cells from Mops condylurus

For more than 40 years, outbreaks of ebolavirus disease have been documented, but the natural reservoir(s) of ebolaviruses remain unknown. However, recent studies provide evidence that the Angolan free-tailed bat (Mops condylurus), an insectivorous bat belonging to the family Molossidae, is a likely ebolavirus reservoir.Being a heterothermic species, M. condylurus bats are highly tolerant to variations in ambient temperatures, and therefore are capable of living under a broad range of climatic and environmental conditions by using adaptive thermoregulation. Body core temperatures as low as 12.0°C have been measured during winter, while increased body temperatures were observed in their hot roost or during flight, reaching temperatures typical for fever in most other mammalian species.Here we investigated the impacts of temperature fluctuations between 27°C and 42°C on Ebola virus (EBOV) survival and replication kinetics in cells from M. condylurus using qRT-PCR.We found that primary cells derived from M. condylurus, similar to the bats in their natural environment, were highly tolerant to temperature variations. EBOV replication was temperature-dependent, showing a strong reduction of replication efficiency at low temperature. We therefore conclude, that heterothermy might be involved in balancing the level of EBOV replication and thereby be a potential key factor for tolerating EBOV infections in vivo.