Article CC BY 4.0
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High-resolution mapping of Ryd4Hb, a major resistance gene to Barley yellow dwarf virus from Hordeum bulbosum

GND
1254993622
ORCID
0000-0002-9802-1787
Affiliation
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Seeland, Germany; IPSiM, Univ Montpellier, CNRS, INRAE, Institut Agro, Montpellier, France
Pidon, Hélène;
GND
115742360
Affiliation
Julius Kühn Institute (JKI), Institute for Breeding Research on Agricultural Crops, Germany
Ruge-Wehling, Brigitte;
GND
137978146
ORCID
0000-0001-7985-4292
Affiliation
Julius Kühn Institute (JKI), Institute for Resistance Research and Stress Tolerance, Germany
Will, Torsten;
GND
1059141299
ORCID
0000-0001-9627-0280
Affiliation
Julius Kühn Institute (JKI), Institute for Resistance Research and Stress Tolerance, Germany
Habekuß, Antje;
Affiliation
KWS SAAT SE & Co. KGaA, Einbeck, Germany
Wendler, Neele;
GND
122024435
ORCID
0000-0002-7114-2650
Affiliation
KWS LOCHOW GMBH, Bergen, Germany
Oldach, Klaus;
Affiliation
KWS LOCHOW GMBH, Bergen, Germany
Maasberg-Prelle, Anja;
GND
1177839385
ORCID
0000-0003-0760-8249
Affiliation
KWS SAAT SE & Co. KGaA, Einbeck, Germany
Korzun, Viktor;
GND
120077000
ORCID
0000-0003-3011-8731
Affiliation
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Seeland, Germany; Center for Integrated Breeding Research (CiBreed), Georg-August University, Göttingen, Germany
Stein, Nils

Key message: We mapped Ryd4Hb in a 66.5 kbp interval in barley and dissociated it from a sublethality factor. These results will enable a targeted selection of the resistance in barley breeding.
Abstract: Virus diseases are causing high yield losses in crops worldwide. The Barley yellow dwarf virus (BYDV) complex is responsible for one of the most widespread and economically important viral diseases of cereals. While no gene conferring complete resistance (immunity) has been uncovered in the primary gene pool of barley, sources of resistance were searched and identified in the wild relative Hordeum bulbosum, representing the secondary gene pool of barley. One such locus, Ryd4Hb, has been previously introgressed into barley, and was allocated to chromosome 3H, but is tightly linked to a sublethality factor that prevents the incorporation and utilization of Ryd4Hb in barley varieties. To solve this problem, we fine-mapped Ryd4Hb and separated it from this negative factor. We narrowed the Ryd4Hb locus to a corresponding 66.5 kbp physical interval in the barley 'Morex' reference genome. The region comprises a gene from the nucleotide-binding and leucine-rich repeat immune receptor family, typical of dominant virus resistance genes. The closest homolog to this Ryd4Hb candidate gene is the wheat Sr35 stem rust resistance gene. In addition to the fine mapping, we reduced the interval bearing the sublethality factor to 600 kbp in barley. Aphid feeding experiments demonstrated that Ryd4Hb provides a resistance to BYDV rather than to its vector. The presented results, including the high-throughput molecular markers, will permit a more targeted selection of the resistance in breeding, enabling the use of Ryd4Hb in barley varieties.

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License Holder: The Author(s) 2024

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