Unexpected microbial metabolic responses to elevated temperatures and nitrogen addition in subarctic soils under different land-use
This study investigated how subarctic soils under different land use will respond to warming and increasing N availability to allow for better predictions of C cycling under global change. The short-term temperature sensitivity as well as N-input effects on microbial CUE, respiration, growth and turnover were assessed in a one-day incubation experiment according to the 18O-CUE approach. The warming and N response of SOM decomposition were assessed in a 50-days incubation experiment via measurement of cumulative respiration. Both experiments were conducted with the following three treatments: incubation at 10 °C, incubation at 20 °C, and incubation at 20 °C plus N-fertiliser addition at an amendment rate of 100 kg N ha-1. The response to warming or N addition were expressed as response ratios RRT = 20°C/10°C and RRN = 20°C+N/20°C for warming and N response, respectively. The R code was developed under R v3.6.3 and adapted to work under version R v.4.1.2. The repository includes the following files: This study was part of the "Breaking the Ice" project funded by the German Research Foundation (DFG) [grant number 401106790]. Funding for E.G. was provided by the Science & Technology Branch of Agriculture & Agri-Food Canada [Project J-001756 'Biological Soil Carbon Stabilisation'].