Tissue optical clearing and 3D imaging of virus infections

GND
1239437080
Affiliation
Institute for Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Greifswald-Insel, Riems, Germany
Ushakov, Dmitry S.;
GND
1019541385
Affiliation
Institute for Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Greifswald-Insel, Riems, Germany
Finke, Stefan

Imaging pathogens within 3D environment of biological tissues provides spatial information about their localization and interactions with the host. Technological advances in fluorescence microscopy and 3D image analysis now permit visualization and quantification of pathogens directly in large tissue volumes and in great detail. In recent years large volume imaging became an important tool in virology research helping to understand the properties of viruses and the host response to infection. In this chapter we give a review of fluorescence microscopy modalities and tissue optical clearing methods used for large volume tissue imaging. A summary of recent applications for virus research is provided with particular emphasis on studies using light sheet fluorescence microscopy. We describe the challenges and approaches for volumetric image analysis. Practical examples of volumetric imaging implemented in virology laboratories and addressing specialized research questions, such as virus tropism and immune host response are described. We conclude with an overview of the emerging technologies and their potential for virus research.

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