Ecotoxicological methods to evaluate the toxicity of bio-based fertilizer application to agricultural soils – A review

A multitude of possible contaminants can be contained in bio-based fertilizers (BBFs) because of their complex matrix. The chemical characterization of BBFs is a challenging analytical task. Therefore, it is important for sustainable agricultural production to develop standard procedures to assess new bio-based fertilizers for possible hazards related to their application in order to guarantee their safety for soils organisms, plants and the environment.

There is a huge number of ecotoxicological tests for aquatic and terrestrial organisms. They were developed for the evaluation of chemicals, pesticides and industrial wastes on aquatic systems and soil functioning. These tests can be useful for the assessment of BBFs. Ecotoxicological tests in comparison to chemical analysis have the advantage to capture the effects of all possible contaminants and metabolites available in the product. The bioavailability of toxic compounds and their interaction are recorded while the cause-and-effect-chain is not elucidated. Numerous ecotoxicological tests work with liquid media, capturing the effects of pollutants that can be mobilized. Hence, standardized procedures how to produce solvents from BBFs are mandatory. Moreover, tests using the original (solid) material are necessary in order to determine the toxicity of a given BBF in its application form and to cover the potential toxicity of non-soluble compounds. To date there are no rules how to determine the ecotoxicological potential of BBFs. A tiered approach of chemical analytical parameters in combination with a set of ecotoxicological tests and the measurement of sensitive soil indicators seem to be a promising experimental setup for the evaluation of BBFs. A decision tree for such an approach was developed. An extended ecotoxicological test strategy of BBFs is mandatory to identify the most promising raw materials and BBF processing technologies to end up with sustainable fertilizer products showing a high agronomic efficiency.