Article CC BY 4.0
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Elucidating the genomic history of commercially used Bacillus thuringiensis subsp. tenebrionis strain NB176

GND
1242996451
Affiliation
Julius Kühn-Institute (JKI), Institute for Biological Control, Germany
Schäfer, Lea;
Affiliation
Biofa GmbH, Germany
Volk, Frank;
GND
1059093685
Affiliation
Julius Kühn-Institute (JKI), Institute for Biological Control, Germany
Kleespies, Regina G.;
GND
17274184X
Affiliation
Julius Kühn-Institute (JKI), Institute for Biological Control, Germany
Jehle, Johannes A.;
GND
1059101742
Affiliation
Julius Kühn-Institute (JKI), Institute for Biological Control, Germany
Wennmann, Jörg T.

Bacillus thuringiensis subsp. tenebrionis (Btt) produces a coleopteran-specific crystal protoxin protein (Cry3Aa δ-endotoxin). After its discovery in 1982, the strain NB125 (DSM 5526) was eventually registered in 1990 to control the Colorado potato beetle (Leptinotarsa decemlineata). Gamma-irradiation of NB125 resulted in strain NB176-1 (DSM 5480) that exhibited higher cry3Aa production and became the active ingredient of the plant protection product Novodor® FC. Here, we report a comparative genome analysis of the parental strain NB125, its derivative NB176-1 and the current commercial production strain NB176. The entire genome sequences of the parental and derivative strains were deciphered by a hybrid de novo approach using short (Illumina) and long (Nanopore) read sequencing techniques. Genome assembly revealed a chromosome of 5.4 to 5.6 Mbp and six plasmids with a size range from 14.9 to 250.5 kbp for each strain. The major differences among the original NB125 and the derivative strains NB176-1 and NB176 were an additional copy of the cry3Aa gene, which translocated to another plasmid as well as a chromosomal deletion (~ 178 kbp) in NB176. The assembled genome sequences were further analyzed in silico for the presence of virulence and antimicrobial resistance (AMR) genes.

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License Holder: 2023 Schäfer, Volk, Kleespies, Jehle and Wennmann.

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