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Encapsulation of the psyllid‐pathogenic fungus Pandora sp. nov. inedit. and experimental infection of target insects

Affiliation
Bielefeld University of Applied Sciences, Faculty of Engineering and Mathematics, WG Fermentation and Formulation of Biologicals and Chemicals, Interaktion 1, D-33619 Bielefeld, Germany; Georg-August-University Göttingen, Agricultural Entomology, Department of Crop Sciences, Göttingen, Germany
Muskat, Linda C.;
GND
1172233233
Affiliation
Julius Kühn-Institute (JKI), Institute for Plant Protection in Fruit Crops and Viticulture, Germany
Görg, Louisa M.;
Affiliation
Bielefeld University of Applied Sciences, Faculty of Engineering and Mathematics, WG Fermentation and Formulation of Biologicals and Chemicals, Interaktion 1, D-33619 Bielefeld, Germany
Humbert, Pascal;
GND
1059102293
Affiliation
Julius Kühn-Institute (JKI), Institute for Plant Protection in Fruit Crops and Viticulture, Germany
Gross, Jürgen;
GND
1177696010
Affiliation
University of Copenhagen, Department of Plant and Environmental Sciences, Thorvaldsensvej 40, 1871 Frb. C., Denmark
Eilenberg, Jørgen;
Affiliation
Bielefeld University of Applied Sciences, Faculty of Engineering and Mathematics, WG Fermentation and Formulation of Biologicals and Chemicals, Interaktion 1, D-33619 Bielefeld, Germany
Patel, Anant V.

BACKGROUND: Pandora sp. nov. inedit. (Entomophthorales: Entomophthoraceae) (ARSEF 13372) is a recently isolated entomophthoralean fungus with potential for psyllid pest control. This study aimed to develop a formulation based on biocompatible hydrogel beads in order to transfer the fungus into an easily applicable form and to test the effects on psyllids. RESULTS: After encapsulation in calcium alginate beads, Pandora sp. nov. grew from the beads and discharged conidia over 12 days under optimal humidity conditions at 18 °C. Conidial number was increased 2.95-fold by the addition of skimmed milk as nutritional formulation adjuvant to the beads. The virulence of the encapsulated fungus was assessed with the two target psyllid species; the summer apple psyllid, Cacopsylla picta and the pear psyllid, Cacopsylla pyri. Beads containing skimmed milk as nutritional adjuvant led to the highest mortalities (48.3% on C. picta and 75.0 % on C. pyri). In a second bioassay, survival time of C. pyri exposed to beads containing different concentration (10, 20 or 40%) of Pandora sp. nov. was tested. The survival time of C. pyri was significantly reduced when exposed to beads containing 10% or 20% Pandora sp. nov. The median survival time was reached after 5-6 days past inoculation and the cumulative mortality for C. pyri treated with Pandora sp. nov. beads showed up to 89% mortality. CONCLUSION: The promising results of this study will ease the way for large-scale field application of a novel Pandora species in biological psyllid pest control.

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License Holder: 2021. The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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