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甘蓝型油菜- 萝卜C 染色体附加系与普通 白菜杂交F1 的SSR 标记鉴定 = Identification of F1 Hybrid Derived from Crossing Rape-radish Chromos Addition Line and Pakchoi by SSR Molecular Markers

Affiliation
Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing 100097, China
Ding, Yunhua;
GND
1059150301
Affiliation
Julius Kühn-Institute (JKI), Institute for Breeding Research on Horticultural Crops, Germany
Budahn, Holger;
Affiliation
Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing 100097, China
Zhao, Hong;
Affiliation
Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing 100097, China
Zhao, Xiuyun

According to 21 standard materials with known radish-chromosome information in the genetic background, two SSR molecular markers RsSA078-170 and RH148-190, which were specific for radish chromosome C, were confirmed. Both SSR markers were used to identify the alien radish chromosome C in the genetic backround of hybrid F1 being derived from the cross of rape-radish chromosome C addition line and pakchoi ‘559’. The result showed that all individuals of F1 were amplified with the specific bands of radish chromosome C. Meanwhile, the radish genomic probe pURsN was used to detect the radish genomoe of F1 by fluorescence in situ hybridization technology. The result showed that F1 had hybridization signal indicating that F1 was added with alien radish chromosome, which proved the accuracy of the identification results of two SSR markers RsSA078-170 and RH148-190. It showed that both SSR markers could be used to identify the radish chromosome C in the progeny of rape-radish chromosome C addition line and pakchoi.

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