In vitro assessment of enzymatic phytate dephosphorylation during digestive process of different feeds and feed ingredients

Affiliation
Univ Rennes, Ecole Nationale Supérieure de Chimie de Rennes, CNRS, ISCR - UMR 6226, Rennes, France
Riviere, Arnaud;
Affiliation
Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Haid-und-Neu-Straße 9, Karlsruhe, Germany
Nothof, Thomas;
GND
1036650022
Affiliation
Max Rubner-Institut (MRI), Federal Research Institute of Nutrition and Food, Department of Food Technology and Bioprocess Engineering, Germany
Greiner, Ralf;
Affiliation
Univ Rennes, Ecole Nationale Supérieure de Chimie de Rennes, CNRS, ISCR - UMR 6226, Rennes, France
Tranchimand, S.;
Affiliation
Univ Rennes, Ecole Nationale Supérieure de Chimie de Rennes, CNRS, ISCR - UMR 6226, Rennes, France
Noiret, N.;
Affiliation
CCPA Group, Z.A. du Bois de Teillay, Quartier du Haut-Bois, Janzé, France
Robert, F.;
Affiliation
CCPA Group, Z.A. du Bois de Teillay, Quartier du Haut-Bois, Janzé, France
Mireaux, Melanie

In vivo studies of the digestive process are long, expensive and difficult to rationalize, whereas in vitro systems may give more accessible insight into parts of this process. The purpose of this study was to show the ability of a three-step simulation of monogastric animals’ digestive system to estimate phytate hydrolysis and how it is affected by feed composition. Several feed ingredients: wheat, maize, soybean meal and rapeseed meal and complete diets: a wheat-maize-soybean-meal-based diet, a maize-soybean-meal diet and a wheat-maize-rapeseed-meal diet were treated using an adaptation of a described in vitro digestion simulation system in the presence of increasing doses of phytase. A strong dependence of phytate hydrolysis on the feed ingredient used was obtained: phosphorus releases were 0.3, 0.8, 1.0 and 1.6 g/kg at 0 U/kg of phytase supplementation for maize, soybean meal, wheat and rapeseed meal respectively and 1.2, 2.9, 1.7 and 3.9 at 1000 U/kg of bacterial phytase. The efficacy of enzymatic dephosphorylation of phytate was found dependent on the ingredient, which can be partially explained by their initial content in myo-inositol phosphates. The in vitro simulation was proven a useful tool to assess enzymatic dephosphorylation of phytate under different conditions.

Preview

Cite

Citation style:
Could not load citation form.

Access Statistic

Total:
Downloads:
Abtractviews:
Last 12 Month:
Downloads:
Abtractviews:

Rights

Use and reproduction:
All rights reserved