Palm foliage as pathways of pathogenic Botryosphaeriaceae fungi and host of new Lasiodiplodia species from Mexico

Tropical palm foliage is increasingly imported to satisfy the steady growing demand in European floristry. This palm foliage presumably carries along diverse fungi whose taxonomic and functional diversity have not been addressed so far. The present study investigated Botryosphaeriaceae fungi associated with the foliage of palm species Chamaedorea elegans, C. metallica, C. seifrizii, Dypsis lutescens and Lodoicea maldivica imported from Mexico. Five species were identified using combined morphological characterisation and multilocus phylogenetic analyses based on ITS, TEF-1α, TUB2 and RPB2. In addition to Endomelanconiopsis endophytica, Lasiodiplodia brasiliensis and L. euphorbicola, two new species, namely, L. lodoiceae sp. nov. and L. mexicanensis sp. nov, are proposed. Apart from E. endophytica, mostly known as endophyte, L. brasiliensis and L. euphorbicola are responsible for different rot diseases and the dieback of important tropical crop plants. In pathogenicity tests on the temperate pome fruits apple (Malus domestica) and pear (Pyrus communis), all six Botryosphaeriaceae species induced necrotic lesions at different degrees of severity, with highest the aggressiveness from L. euphorbicola and L. mexicanensis on apple and from L. mexicanensis on pear. The results indicate that tropical palm foliage can be a pathway of potentially pathogenic fungi that may give rise to concerns with regard to plant health in the destination countries.