Article CC BY 4.0
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Cross-Resistance of the Codling Moth against Different Isolates of Cydia pomonella Granulovirus Is Caused by Two Different but Genetically Linked Resistance Mechanisms

GND
1059093855
Affiliation
Julius Kühn-Institute (JKI), Institute for Biological Control, Germany
Sauer, Annette J.;
GND
1059093111
Affiliation
Julius Kühn-Institute (JKI), Institute for Biological Control, Germany
Fritsch, Eva;
GND
172741726
Affiliation
Julius Kühn-Institute (JKI), Institute for Biological Control, Germany
Undorf-Spahn, Karin;
Affiliation
Tokyo University of Agriculture and Technology, Department of Applied Biological Science, Faculty of Agriculture, Japan
Iwata, Kento;
GND
1059093685
Affiliation
Julius Kühn-Institute (JKI), Institute for Biological Control, Germany
Kleespies, Regina G.;
Affiliation
Tokyo University of Agriculture and Technology, Department of Applied Biological Science, Faculty of Agriculture, Japan
Nakai, Madoka;
GND
17274184X
Affiliation
Julius Kühn-Institute (JKI), Institute for Biological Control, Germany
Jehle, Johannes A.

Cydia pomonella granulovirus (CpGV) is a widely used biological control agent of the codling moth. Recently, however, the codling moth has developed different types of field resistance against CpGV isolates. Whereas type I resistance is Z chromosomal inherited and targeted at the viral gene pe38 of isolate CpGV-M, type II resistance is autosomal inherited and targeted against isolates CpGV-M and CpGV-S. Here, we report that mixtures of CpGV-M and CpGV-S fail to break type II resistance and is expressed at all larval stages. Budded virus (BV) injection experiments circumventing initial midgut infection provided evidence that resistance against CpGV-S is midgutrelated, though fluorescence dequenching assay using rhodamine-18 labeled occlusion derived viruses (ODV) could not fully elucidate whether the receptor binding or an intracellular midgut factor is involved. From our peroral and intra-hemocoel infection experiments, we conclude that two different (but genetically linked) resistance mechanisms are responsible for type II resistance in the codling moth: resistance against CpGV-M is systemic whereas a second and/or additional resistance mechanism against CpGV-S is located in the midgut of CpR5M larvae.

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