Clinico-hematological, patho-anatomical and molecular based investigation of blackleg disease in Cholistani cattle

Affiliation
Department of Pathology, Faculty of Veterinary and Animal Sciences, The Islamia University of, Bahawalpur, Pakistan
Hussain, Riaz;
Affiliation
Department of Pathobiology, University of Veterinary and Animal Sciences, Sub-Campus, Jhang, Lahore, Pakistan
Ehtisham-Ul-haque, Syed;
Affiliation
Department of Pathobiology, University of Veterinary and Animal Sciences, Sub-Campus, Jhang, Lahore, Pakistan
Khan, Iahtasham;
Affiliation
Department of Zoology, Lahore College for Women University, Lahore, Pakistan
Jabeen, Ghazala;
Affiliation
Department of Microbiology, Faculty of Science and Technology, Government College University, Faisalabad, Pakistan
Siddique, Abu Baker;
Affiliation
Department of Pathology, Faculty of Veterinary and Animal Sciences, The Islamia University of, Bahawalpur, Pakistan
Ghaffar, Abdul;
Affiliation
Department of Parasitology, Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan
Abbas, Rao Zahid;
Affiliation
Faculty of Veterinary and Animal Sciences, Muhammad Nawaz Shareef University of Agriculture, Multan, Pakistan
Ishaq, Hafiz Muhammad;
Affiliation
Department of Parasitology, Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan
Javed, Muhammad Tariq;
Affiliation
Department of Pathobiology, University of Veterinary and Animal Sciences, Sub-Campus, Jhang, Lahore, Pakistan
Tariq, Narmeen;
Affiliation
Department of Pathology, Faculty of Veterinary and Animal Sciences, The Islamia University of, Bahawalpur, Pakistan
Ghori, Muhammad Taslim;
Affiliation
Department of Pathology, Faculty of Veterinary and Animal Sciences, The Islamia University of, Bahawalpur, Pakistan
Qayyum, Abdul;
Affiliation
Faculty of Veterinary and Animal Sciences, Muhammad Nawaz Shareef University of Agriculture, Multan, Pakistan
Abbas, Asghar;
Affiliation
Department of Pathobiology, University of Veterinary and Animal Sciences, Sub-Campus, Jhang, Lahore, Pakistan
Jalees, Muhammad Moazzam;
GND
1060328526
Affiliation
Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh, Egypt
Wareth, Gamal

Clostridium chauvoei, causing myonecrosis in livestock animals, lives in the feces, surface water and soil. The blackleg due to C. chauvoei is very common in dairy animals. Still, no report is available about the pathophysiology of disease in Cholistani cattle kept under tropical and desert conditions of Cholistan, Pakistan. Therefore, in this study, we report for the first time the pathophysiology of a visceral form of blackleg infection in indigenous cholistani cattle breed reared in desert conditions of Cholistan. Clinically morbid animals exhibited different signs of infection including fever, crepitation sounds, gaseous swelling and edematous lesions. Blood was collected for hematological and serum biochemical investigation. Hematological examination indicated a significant increased erythrocyte sedimentation rate, lower red blood cells, hematocrit, and total white blood cell count. Results on serum biochemistry showed significantly (p<0.02) increased creatine kinase levels, creatinine, urea, alanine aminotransferase, aspartate aminotransferase, alkaline phosphates, creatinine phosphokinase and lactate dehydrogenase in infected cattle. At necropsy level, light pink color fluid under swelling areas of skin, gas bubbles, dark to black in color of affected muscles and crepitation sounds at palpation were observed. Necropsy showed marked myocarditis, petechial hemorrhages, consolidation and severe pulmonary edema. Spleen showed petechial hemorrhages and congestion. Histological analysis of muscular tissues indicated severe inflammatory reaction comprising of cellular infiltration, marked edema, necrosis and disruption of myofibrils. PCR confirmed the presence of C. chauvoei in muscles, heart and exudates of the lungs. This is the first report on molecular detection of Clostridium chauvoei from a visceral form of Blackleg disease in cholistani breed of cattle naturally infected in the Cholistan desert of Punjab, Pakistan.

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