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Identification of a blaVIM₋₁-carrying inca/c₂ multiresistance plasmid in an escherichia coli isolate recovered from the german food chainIdentification of a blaVIM-1-Carrying IncA/C2 Multiresistance Plasmid in an Escherichia coli Isolate Recovered from the German Food Chain

Affiliation
Department Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany
Pauly, N.;
ORCID
0000-0002-6930-4358
Affiliation
German Federal Institute for Risk Assessment (BfR), Department 4 Biological Safety, Unit 43 Epidemiology, Zoonoses and Antimicrobial Resistance, Germany
Hammerl, Jens Andre;
ORCID
0000-0002-8619-1498
Affiliation
German Federal Institute for Risk Assessment (BfR), Department 4 Biological Safety, Unit 43 Epidemiology, Zoonoses and Antimicrobial Resistance, Germany
Grobbel, Mirjam;
Affiliation
German Federal Institute for Risk Assessment (BfR), Department 4 Biological Safety, Unit 43 Epidemiology, Zoonoses and Antimicrobial Resistance, Germany
Käsbohrer, Annemarie;
ORCID
0000-0002-2191-7569
Affiliation
German Federal Institute for Risk Assessment (BfR), Department 4 Biological Safety, Unit 43 Epidemiology, Zoonoses and Antimicrobial Resistance, Germany
Tenhagen, Bernd-Alois;
Affiliation
German Federal Institute for Risk Assessment (BfR), Department 4 Biological Safety, Study Centre 4SZ - Genome Sequencing and Analysis, Germany
Malorny, Burkhard;
Affiliation
Institute of Microbiology and Epizootics, Freie Universität Berlin, Berlin, Germany
Schwarz, S.;
Affiliation
Institute of Microbiology and Epizootics, Freie Universität Berlin, Berlin, Germany
Meemken, D.;
ORCID
0000-0003-1266-9978
Affiliation
German Federal Institute for Risk Assessment (BfR), Department 4 Biological Safety, Unit 43 Epidemiology, Zoonoses and Antimicrobial Resistance, Germany
Irrgang, Alexandra

Within the German national monitoring of zoonotic agents, antimicrobial resistance determination also targets carbapenemase-producing (CP) Escherichia coli by selective isolation from food and livestock. In this monitoring in 2019, the CP E. coli 19-AB01133 was recovered from pork shoulder. The isolate was assigned to the phylogenetic group B1 and exhibited the multi-locus sequence-type ST5869. Molecular investigations, including whole genome sequencing, of 19-AB01133 revealed that the isolate carried the resistance genes blaVIM₋₁, blaSHV₋₅ and blaCMY₋₁₃ on a self-transmissible IncA/C₂ plasmid. The plasmid was closely related to the previously described VIM-1-encoding plas-mid S15FP06257_p from E. coli of pork origin in Belgium. Our results indicate an occasional spread of the blaVIM₋₁ gene in Enterobacteriaceae of the European pig population. Moreover, the blaVIM₋₁ located on an IncA/C₂ plasmid supports the presumption of a new, probably human source of carbapenemase-producing Enterobacteriaceae (CPE) entering the livestock and food chain sector.

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