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Genome-Wide Approach to Identify Quantitative Trait Loci for Drought Tolerance in Tetraploid Potato (Solanum tuberosum L.)

Affiliation
University of Rostock, Department of Plant Genetics, Institute of Biological Sciences, Germany
Schumacher, Christina;
Affiliation
University of Rostock, Department of Plant Genetics, Institute of Biological Sciences, Germany
Thümecke, Susanne;
Affiliation
University of Rostock, Department of Plant Genetics, Institute of Biological Sciences, Germany
Schilling, Florian;
Affiliation
Max Planck Institute of Molecular Plant Physiology, Germany
Köhl, Karin;
Affiliation
Max Planck Institute of Molecular Plant Physiology, Germany
Kopka, Joachim;
Affiliation
Max Planck Institute of Molecular Plant Physiology, Germany
Sprenger, Heike;
Affiliation
Max Planck Institute of Molecular Plant Physiology, Germany
Hincha, Dirk Karl;
Affiliation
Max Planck Institute of Molecular Plant Physiology, Germany
Walther, Dirk;
GND
105914123X
Affiliation
Julius Kühn-Institute (JKI), Institute for Resistance Research and Stress Tolerance, Germany
Seddig, Sylvia;
Affiliation
Landwirtschaftskammer Niedersachsen, Germany
Peters, Rolf;
Affiliation
Max Planck Institute of Molecular Plant Physiology, Germany
Zuther, Ellen;
Affiliation
Max Planck Institute of Molecular Plant Physiology, Germany
Haas, Manuela;
Affiliation
University of Rostock, Department of Plant Genetics, Institute of Biological Sciences, Germany
Horn, Renate

Drought represents a major abiotic stress factor negatively affecting growth, yield and tuber quality of potatoes. Quantitative trait locus (QTL) analyses were performed in cultivated potatoes for drought tolerance index DRYM (deviation of relative starch yield from the experimental median), tuber starch content, tuber starch yield, tuber fresh weight, selected transcripts and metabolites under control and drought stress conditions. Eight genomic regions of major interest for drought tolerance were identified, three representing standalone DRYM QTL. Candidate genes, e.g., from signaling pathways for ethylene, abscisic acid and brassinosteroids, and genes encoding cell wall remodeling enzymes were identified within DRYM QTL. Co-localizations of DRYM QTL and QTL for tuber starch content, tuber starch yield and tuber fresh weight with underlying genes of the carbohydrate metabolism were observed. Overlaps of DRYM QTL with metabolite QTL for ribitol or galactinol may indicate trade-offs between starch and compatible solute biosynthesis. Expression QTL confirmed the drought stress relevance of selected transcripts by overlaps with DRYM QTL. Bulked segregant analyses combined with next-generation sequencing (BSAseq) were used to identify mutations in genes under the DRYM QTL on linkage group 3. Future analyses of identified genes for drought tolerance will give a better insight into drought tolerance in potatoes.

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