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Transcriptional profile of AvrRpt2EA-mediated resistance and susceptibility response to Erwinia amylovora in apple

GND
1024798933
Affiliation
Julius Kühn-Institute (JKI), Institute for Breeding Research on Fruit Crops, Germany
Schröpfer, Susan;
GND
1156465087
Affiliation
Julius Kühn-Institute (JKI), Institute for Breeding Research on Fruit Crops, Germany
Vogt, Isabelle;
Affiliation
ETH Zurich, Molecular Plant Breeding, Institute of Agricultural Sciences, Switzerland
Broggini, Giovanni Antonio Lodovico;
Affiliation
Technische Universität Dresden, DRESDEN‑Concept Genome Center, Center for Molecular and Cellular Bioengineering (CMCB), Germany
Dahl, Andreas;
GND
1059141744
Affiliation
Julius Kühn-Institute (JKI), Institute for Resistance Research and Stress Tolerance, Germany
Richter, Klaus;
GND
1059103400
Affiliation
Julius Kühn-Institute (JKI), Institute for Breeding Research on Fruit Crops, Germany
Hanke, Magda-Viola;
GND
128593652
Affiliation
Julius Kühn-Institute (JKI), Institute for Breeding Research on Fruit Crops, Germany
Flachowsky, Henryk;
GND
172861896
Affiliation
Julius Kühn-Institute (JKI), Institute for Breeding Research on Fruit Crops, Germany
Peil, Andreas

Most of the commercial apple cultivars are highly susceptible to fire blight, which is the most devastating bacterial disease affecting pome fruits. Resistance to fire blight is described especially in wild Malus accessions such as M. × robusta 5 (Mr5), but the molecular basis of host resistance response to the pathogen Erwinia amylovora is still largely unknown. The bacterial effector protein AvrRpt2EA was found to be the key determinant of resistance response in Mr5. A wild type E. amylovora strain and the corresponding avrRpt2EA deletion mutant were used for inoculation of Mr5 to induce resistance or susceptible response, respectively. By comparison of the transcriptome of both responses, 211 differentially expressed genes (DEGs) were identified. We found that heat-shock response including heat-shock proteins (HSPs) and heat-shock transcription factors (HSFs) are activated in apple specifically in the susceptible response, independent of AvrRpt2EA. Further analysis on the expression progress of 81 DEGs by high-throughput real-time qPCR resulted in the identification of genes that were activated after inoculation with E. amylovora. Hence, a potential role of these genes in the resistance to the pathogen is postulated, including genes coding for enzymes involved in formation of flavonoids and terpenoids, ribosome-inactivating enzymes (RIPs) and a squamosa promoter binding-like (SPL) transcription factor.

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