Development of monoclonal neutralizing antibodies against bovine viral diarrhea virus after pretreatment of mice with normal bovine cells and cyclophosphamide

After immunization of mice with bovine viral diarrhea virus (BVDV) concentrated by ultracentrifugation only antibodies against normal cellular constituents were detectable. Monoclonal and polyclonal anti-BVDV antibodies were obtained, however, after induction of tolerance against normal bovine cellular antigens by pretreatment of adult mice with bovine cells and cyclophosphamide followed by immunization with BVDV containing material. © 1989.