Article CC BY 4.0
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Unravelling Differences in Candidate Genes for Drought Tolerance in Potato (Solanum tuberosum L.) by Use of New Functional Microsatellite Markers

Affiliation
University of Rostock, Institute of Biological Sciences, Plant Genetics, Germany
Schumacher, Christina;
Affiliation
University of Rostock, Institute of Biological Sciences, Plant Genetics, Germany
Krannich, Christoph Tim;
Affiliation
University of Rostock, Institute of Biological Sciences, Plant Genetics, Germany
Maletzki, Lisa;
Affiliation
MPI für Molekulare Pflanzenphysiologie, Germany
Köhl, Karin;
Affiliation
MPI für Molekulare Pflanzenphysiologie, Germany
Kopka, Joachim;
Affiliation
MPI für Molekulare Pflanzenphysiologie, Germany ; German Federal Institute for Risk Assessment (BfR), Department of Food Safety, Germany
Sprenger, Heike;
Affiliation
Deceased, Formerly MPI für Molekulare Pflanzenphysiologie, Germany
Hincha, Dirk Karl;
GND
105914123X
Affiliation
Julius Kühn-Institute (JKI), Institute for Resistance Research and Stress Tolerance, Germany
Seddig, Sylvia;
Affiliation
Landwirtschaftskammer Niedersachsen, Germany ; PotatoConsult UG, Germany
Peters, Rolf;
Affiliation
University of Rostock, Institute of Biological Sciences, Plant Genetics, Germany
Hamera, Sadia;
Affiliation
MPI für Molekulare Pflanzenphysiologie, Germany
Zuther, Ellen;
Affiliation
MPI für Molekulare Pflanzenphysiologie, Germany ; Ministry of Agriculture, Environment and Climate Protection, Germany
Haas, Manuela;
Affiliation
University of Rostock, Institute of Biological Sciences, Plant Genetics, Germany
Horn, Renate

Potato is regarded as drought sensitive and most vulnerable to climate changes. Its cultivation in drought prone regions or under conditions of more frequent drought periods, especially in subtropical areas, requires intensive research to improve drought tolerance in order to guarantee high yields under limited water supplies. A candidate gene approach was used to develop functional simple sequence repeat (SSR) markers for association studies in potato with the aim to enhance breeding for drought tolerance. SSR primer combinations, mostly surrounding interrupted complex and compound repeats, were derived from 103 candidate genes for drought tolerance. Validation of the SSRs was performed in an association panel representing 34 mainly starch potato cultivars. Seventy-five out of 154 SSR primer combinations (49%) resulted in polymorphic, highly reproducible banding patterns with polymorphic information content (PIC) values between 0.11 and 0.90. Five SSR markers identified allelic differences between the potato cultivars that showed significant associations with drought sensitivity. In all cases, the group of drought-sensitive cultivars showed predominantly an additional allele, indicating that selection against these alleles by marker-assisted breeding might confer drought tolerance. Further studies of these differences in the candidate genes will elucidate their role for an improved performance of potatoes under water-limited conditions.

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License Holder: 2021 by the authors.

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