Article CC BY 4.0
refereed
published

Taraxacum kok-saghyz (rubber dandelion) genomic microsatellite loci reveal modest genetic diversity and cross-amplify broadly to related species

Affiliation
The University of Tennessee, Department of Entomology and Plant Pathology, Knoxville, United States
Nowicki, Marcin;
Affiliation
Guizhou Key Laboratory of Agro-Bioengineering, Guizhou University, Huaxi, China
Zhao, Yichen;
Affiliation
The University of Tennessee, Department of Entomology and Plant Pathology, Knoxville, United States
Boggess, Sarah L.;
GND
1059145707
Affiliation
Julius Kühn-Institute (JKI), Institute for Breeding Research on Agricultural Crops, Germany
Flüß, Helge;
Affiliation
The University of Tennessee, Department of Entomology and Plant Pathology, Knoxville, United States
Payá-Milans, Miriam;
Affiliation
The University of Tennessee, Department of Entomology and Plant Pathology, Knoxville, United States
Staton, Margaret E.;
Affiliation
The University of Tennessee, Department of Entomology and Plant Pathology, Knoxville, United States
Houston, Logan C.;
Affiliation
The University of Tennessee, Department of Entomology and Plant Pathology, Knoxville, United States
Hadziabdic, Denita;
Affiliation
The University of Tennessee, Department of Entomology and Plant Pathology, Knoxville, United States
Trigiano, Robert N.

Taraxacum kok-saghyz (TKS) carries great potential as alternative natural rubber source. To better inform future breeding efforts with TKS and gain a deeper understanding of its genetic diversity, we utilized de novo sequencing to generate novel genomic simple sequence repeats markers (gSSRs). We utilized 25 gSSRs on a collection of genomic DNA (gDNA) samples from germplasm bank, and two gDNA samples from historical herbarium specimens. PCR coupled with capillary electrophoresis and an array of population genetics tools were employed to analyze the dataset of our study as well as a dataset of the recently published genic SSRs (eSSRs) generated on the same germplasm. Our results using both gSSRs and eSSRs revealed that TKS has low- to- moderate genetic diversity with most of it partitioned to the individuals and individuals within populations, whereas the species lacked population structure. Nineteen of the 25 gSSR markers cross-amplified to other Taraxacum spp. collected from Southeastern United States and identified as T. officinale by ITS sequencing. We used a subset of 14 gSSRs to estimate the genetic diversity of the T. officinale gDNA collection. In contrast to the obligatory outcrossing TKS, T. officinale presented evidence for population structure and clonal reproduction, which agreed with the species biology. We mapped the molecular markers sequences from this study and several others to the well-annotated sunflower genome. Our gSSRs present a functional tool for the biodiversity analyses in Taraxacum, but also in the related genera, as well as in the closely related tribes of the Asteraceae.

Preview

Cite

Citation style:
Could not load citation form.

Access Statistic

Total:
Downloads:
Abtractviews:
Last 12 Month:
Downloads:
Abtractviews:

Rights

License Holder: The Author(s) 2019

Use and reproduction: