Nitrite isotope characteristics and associated soil N transformations

Nitrite (NO₂⁻) is a crucial compound in the N soil cycle. As an intermediate of nearly all N transformations, its isotopic signature may provide precious information on the active pathways and processes. NO₂⁻ analyses have already been applied in ¹⁵N tracing studies, increasing their interpretation perspectives. Natural abundance NO₂⁻ isotope studies in soils were so far not applied and this study aims at testing if such analyses are useful in tracing the soil N cycle. We conducted laboratory soil incubations with parallel natural abundance and ¹⁵N treatments, accompanied by isotopic analyses of soil N compounds (NO₃⁻, NO₂⁻, NH₄⁺). The double ¹⁵N tracing method was used as a reference method for estimations of N transformation processes based on natural abundance nitrite dynamics. We obtained a very good agreement between the results from nitrite isotope model proposed here and the ¹⁵N tracing approach. Natural abundance nitrite isotope studies are a promising tool to our understanding of soil N cycling.