Article CC BY 4.0
refereed
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New peptaibiotics and a cyclodepsipeptide from Ijuhya vitellina: Isolation, identification, cytotoxic and nematicidal activities

Affiliation
Department of Microbial Drugs, Helmholtz Centre for Infection Research, Inhoffenstraße 7, Braunschweig, Germany
Moussa, Ashaimaa Y.;
Affiliation
Department of Microbial Drugs, Helmholtz Centre for Infection Research, Inhoffenstraße 7, Braunschweig, Germany
Lambert, Christopher;
Affiliation
Department of Microbial Drugs, Helmholtz Centre for Infection Research, Inhoffenstraße 7, Braunschweig, Germany
Stradal, Theresia E. B.;
GND
1139055240
Affiliation
Julius Kühn-Institute (JKI), Institute for Epidemiology and Pathogen Diagnostics, Germany
Ashrafi, Samad;
GND
12412514X
Affiliation
Julius Kühn-Institute (JKI), Institute for Epidemiology and Pathogen Diagnostics, Germany
Maier, Wolfgang;
Affiliation
Department of Microbial Drugs, Helmholtz Centre for Infection Research, Inhoffenstraße 7, Braunschweig, Germany
Stadler, Marc;
Affiliation
Department of Microbial Drugs, Helmholtz Centre for Infection Research, Inhoffenstraße 7, Braunschweig, Germany
Helaly, Soleiman E.

Fungal associations with nematodes have attracted scientific attention because of the need to develop new biocontrol agents. In this context, Ijuhya vitellina, an antagonistic fungus previously isolated from the plant parasitic cyst nematode Heterodera filipjevi, was selected to carry out an in-depth metabolomic study for its active metabolites. Herein, three new nonapeptide peptaibols with leucinostatin based sequences were isolated and identified by 1, 2D NMR, and HR-ESI-MS-MS. The absolute configuration was assigned based on Marfay’s analysis and Mosher ester formation. The new leucinostatins manifested moderate nematicidal effect against the plant pathogenic nematode Pratylenchus penetrans with LD₉₀ values ranging from 5 to 7 µg/mL. Furthermore, a cyclodepsipeptide, named arthrichitin D, with five amino acid residues attached to a 3-hydroxy-2,4-dimethylhexadeca-4,6-dienoic fatty acid chain was discovered and showed weak nematicidal effect against Caenorhabditis elegans. Chaetoglobosin B and its 19-O-acetyl derivative were also obtained as minor metabolites, and the activity of chaetoglobosin B on the actin cytoskeleton of mammalian cells was assessed.

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