Pseudomonas paracarnis sp. nov., isolated from refrigerated beef

During a project focusing on the diversity of meat microbiota associated with beef ripening, a Pseudomonas strain was isolated exhibiting high 16S rRNA gene sequence similarities (>99 %) to Pseudomonas carnis DSM 107652^T, P. lactis DSM 29167^T, P. paralactis DSM 29164^T and P. azotoformans DSM 18862^T. Phylogenetic analysis of the complete rpoB gene sequences of the isolate V5/DAB/2/5^T indicated a separate branch with about 99.0 % nucleotide identities to the closest relatives P. carnis DSM 107652^T, P. lactis DSM 29167^T and P. paralactis DSM 29164^T, while average nucleotide identities (ANIb) calculated from the draft genomes were 94.8, 94.2 and 90.2 %, respectively. Pairwise genome-to-genome distance calculations (GGDC) resulted in values of 67.7, 63.5 and 45.7 %, respectively, lying below the actual species demarcation line as well. A second isolate, UBT403, was detected some years later by using matrix-assisted laser desorption ionization-time of flight MS of the microbiota of minced beef. The fatty acid profile of V5/DAB/2/5^T consisted of C_16 : 0, summed feature C _16 : 1ω7c/iso-C_15 : 0 2-OH, C_18 : 1ω7c, C_17 : 0 cyclo, C_12 : 0, C_12 : 0 3-OH, C_10 : 0 3-OH and C_12 : 0 2-OH. The major cellular lipids were aminopholipids, phospholipids, phosphatidylethanolamine and phosphatidylglycerol; the major quinone was Q9 with a minor proportion of Q8. Based on phenotypic and chemotaxonomic characterizations, the isolates can be considered as representing a novel species, for which the name Pseudomonas paracarnis sp. nov. is proposed. The type strain is V5/DAB/2/5^T (=DSM 111363^T=LMG 31846^T); a second strain is UBT403 (=DSM 111362=LMG 31847).