Isolation of Bovine Neutrophils by Fluorescence- and Magnetic-Activated Cell Sorting

Rambault,Marion; Borkute, Rachana; Doz-Deblauwe, Emilie; Le-Vern, Yves; Winter, Nathalie; Dorhoi, Anca GND; Remot, Aude

Flow cytometry and magnetic bead technology enable the separation of cell populations with the highest degree of purity. Here, we describe protocols to sort bovine neutrophils from blood, the labeling and sorting, including gating strategies. We also provide advice to preserve neutrophil viability and detail a protocol to measure phagocytosis and oxidative species production.

Cite

Citation style:

Rambault, Marion / Borkute, Rachana / Doz-Deblauwe, Emilie / et al: Isolation of Bovine Neutrophils by Fluorescence- and Magnetic-Activated Cell Sorting. 2020.

Rights

Use and reproduction:
All rights reserved

Export