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refereed
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Spatial and Temporal Evolutionary Patterns in Puumala Orthohantavirus (PUUV) S Segment

GND
1144497760
Affiliation
Friedrich-Loeffler-Institut (FLI), Institute of Novel and Emerging Infectious Diseases, Germany
Binder, Florian;
GND
1059920832
Affiliation
Friedrich-Loeffler-Institut (FLI), Institute of Novel and Emerging Infectious Diseases, Germany
Ryll, René;
GND
1056070455
Affiliation
Friedrich-Loeffler-Institut (FLI), Institute of Novel and Emerging Infectious Diseases, Germany
Drewes, Stephan;
Affiliation
Friedrich-Loeffler-Institut (FLI), Institute of Novel and Emerging Infectious Diseases, Germany
Jagdmann, Sandra;
GND
1058985760
Affiliation
Julius Kühn-Institute (JKI), Institute for Plant Protection in Horticulture and Forests, Germany
Reil, Daniela;
Affiliation
University of Bern, Institute of Ecology and Evolution, Switzerland
Hiltbrunner, Melanie;
GND
1056940166
Affiliation
Friedrich-Loeffler-Institut (FLI), Institute of Novel and Emerging Infectious Diseases, Germany
Rosenfeld, Ulrike;
GND
1172105332
Affiliation
Julius Kühn-Institute (JKI), Institute for Plant Protection in Horticulture and Forests, Germany
Imholt, Christian;
GND
122411307
Affiliation
Julius Kühn-Institute (JKI), Institute for Plant Protection in Horticulture and Forests, Germany
Jacob, Jens;
Affiliation
University of Bern, Institute of Ecology and Evolution, Switzerland
Heckel, Gerald;
GND
1019565543
Affiliation
Friedrich-Loeffler-Institut (FLI), Institute of Novel and Emerging Infectious Diseases, Germany
Ulrich, Rainer

The S segment of bank vole (Clethrionomys glareolus)-associated Puumala orthohantavirus (PUUV) contains two overlapping open reading frames coding for the nucleocapsid (N) and a non-structural (NSs) protein. To identify the influence of bank vole population dynamics on PUUV S segment sequence evolution and test for spillover infections in sympatric rodent species, during 2010–2014, 883 bank voles, 357 yellow-necked mice (Apodemus flavicollis), 62 wood mice (A. sylvaticus), 149 common voles (Microtus arvalis) and 8 field voles (M. agrestis) were collected in Baden-Wuerttemberg and North Rhine-Westphalia, Germany. In total, 27.9% and 22.3% of bank voles were positive for PUUV-reactive antibodies and PUUV-specific RNA, respectively. One of eight field voles was PUUV RNA-positive, indicating a spillover infection, but none of the other species showed evidence of PUUV infection. Phylogenetic and isolation-by-distance analyses demonstrated a spatial clustering of PUUV S segment sequences. In the hantavirus outbreak years 2010 and 2012, PUUV RNA prevalence was higher in our study regions compared to non-outbreak years 2011, 2013 and 2014. NSs amino acid and nucleotide sequence types showed temporal and/or local variation, whereas the N protein was highly conserved in the NSs overlapping region and, to a lower rate, in the N alone coding part.

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