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Quantification of quality parameters for reliable evaluation of green rooibos (Aspalathus linearis)

GND
140592741
Affiliation
Federal Centre for Breeding Research on Cultivated Plants (BAZ), Institute of Plant Analysis, Quedlinburg, Germany
Schulz, Hartwig;
Affiliation
ARC Infruitec-Nietvoorbij, Private Bag X5026, 7599 Stellenbosch, South Africa
Joubert, Elizabeth;
GND
1172522340
Affiliation
Federal Centre for Breeding Research on Cultivated Plants (BAZ), Institute of Plant Analysis, Quedlinburg, Germany
Schütze, Wolfgang

An LC-MS/UV-Vis method was developed for the reliable identification of the most relevant flavonoids occurring in rooibos. The amounts of the dihydrochalcones aspalathin, (20,3,4,40,60-pentahydroxy- 3-C-b-d glucopyranosyldihydrochalcone), nothofagin (20,3,40,60-tetrahydroxy-3-C-b-d-glucopyranosyldihydrochalcone) and other flavonoids were determined in unfermented and fermented samples, collected at different locations in South Africa. Generally, a substantial loss of both dihydrochalcones was observed after the fermentation process. An NIR spectroscopic method was developed to discriminate between unfermented and fermented rooibos and to predict the aspalathin content in unfermented rooibos. The total antioxidative activity, determined according to the ABTS·+ scavenging method, was found to correlate very good with the aspalathin content in the unfermented plant material (R2=0.812).

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