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Rice Dwarf Phytoreovirus Segment S6-Encoded Nonstructural Protein Has a Cell-to-Cell Movement Function

Zugehörigkeit
Natl. Lab. of Protein Engineering, College of Life Sciences, Peking University, China
Li, Yi;
Zugehörigkeit
Plant Biology Division, Samuel Roberts Noble Found., Inc., United States
Bao, Yi M.;
Zugehörigkeit
Natl. Lab. of Protein Engineering, College of Life Sciences, Peking University, China
Wei, Chun H.; Kang, Zhen S.;
Zugehörigkeit
Natl. Lab. of Protein Engineering, College of Life Sciences, Peking University, China
Zhong, Yong W.;
Zugehörigkeit
Natl. Lab. of Protein Engineering, College of Life Sciences, Peking University, China
Mao, Peng;
Zugehörigkeit
Natl. Lab. of Protein Engineering, College of Life Sciences, Peking University, China
Wu, Gang;
Zugehörigkeit
Natl. Lab. of Protein Engineering, College of Life Sciences, Peking University, China
Chen, Zhang L.;
GND
141232439
Zugehörigkeit
Federal Biological Research Centre for Agriculture and Forestry, Institute for Plant Virology, Microbiology and Biosafety
Schiemann, Joachim;
Zugehörigkeit
Plant Biology Division, Samuel Roberts Noble Found., Inc., United States
Nelson, Richard S.

Rice dwarf virus (RDV) is a member of the genus Phytoreovirus, which is composed of viruses with segmented double-stranded RNA genomes. Proteins that support the intercellular movement of these viruses in the host have not been identified. Microprojectile bombardment was used to determine which open reading frames (ORFs) support intercellular movement of a heterologous virus. A plasmid containing an infectious clone of Potato virus X (PVX) defective in cell-to-cell movement and expressing either β-glucuronidase or green fluorescent protein (GFP) was used for cobombardment with plasmids containing ORFs from RDV gene segments S1 through S12 onto leaves of Nicotiana benthamiana. Cell-to-cell movement of the movement-defective PVX was restored by cobombardment with a plasmid containing S6. In the absence of S6, no other gene segment supported movement. Identical results were obtained with Nicotiana tabacum, a host that allows fewer viruses to infect and spread within its tissue. S6 supported the cell-to-cell movement of the movement-defective PVX in sink and source leaves of N. benthamiana. A mutant S6 lacking the translation start codon did not complement the cell-to-cell movement of the movement-defective PVX. An S6 protein product (Pns6)-enhanced GFP fusion was observed near or within cell walls of epidermal cells from N. tabacum. By immunocytochemistry, unfused Pns6 was localized to plasmodesmata in rice leaves infected with RDV. S6 thus encodes a protein with characteristics identical to those of other viral proteins required for the cell-to-cell movement of their genome and therefore is likely required for the cell-to-cell movement of RDV.

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