Article CC BY 4.0
refereed
published

PromA Plasmids Are Instrumental in the Dissemination of Linuron Catabolic Genes Between Different Genera

Affiliation
Department of Biological Oceanography, Leibniz Institute for Baltic Sea Research, Rostock, Germany
Werner, Johannes;
GND
1119102766
Affiliation
Institute for Epidemiology and Pathogen Diagnostics, Julius Kühn-Institut, Federal Research Centre for Cultivated Plants (JKI), Braunschweig, Germany
Nour, Eman;
Affiliation
Bioinformatics Department, Leibniz Institute DSMZ, German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany
Bunk, Boyke;
Affiliation
Bioinformatics Department, Leibniz Institute DSMZ, German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany
Spröer, Cathrin;
GND
1058967878
Affiliation
Institute for Epidemiology and Pathogen Diagnostics, Julius Kühn-Institut, Federal Research Centre for Cultivated Plants (JKI), Braunschweig, Germany
Smalla, Kornelia;
Affiliation
Division of Soil and Water Management, KU Leuven, Leuven, Belgium
Springael, Dirk;
Affiliation
Division of Soil and Water Management, KU Leuven, Leuven, Belgium
Öztürk, Başak

PromA plasmids are broad host range (BHR) plasmids, which are often cryptic and hence have an uncertain ecological role. We present three novel PromA γ plasmids which carry genes associated with degradation of the phenylurea herbicide linuron, two of which originated from unrelated Hydrogenophaga hosts isolated from different environments (pPBL-H3-2 and pBPS33-2), and one (pEN1) which was exogenously captured from an on-farm biopurification system (BPS). Hydrogenophaga sp. plasmid pBPS33-2 carries all three necessary gene clusters (hylA, dca, ccd) determining the three main steps for conversion of linuron to Krebs cycle intermediates, while pEN1 only determines the initial linuron hydrolysis step. Hydrogenophaga sp. plasmid pPBL-H3-2 exists as two variants, both containing ccd but with the hylA and dca gene modules interchanged between each other at exactly the same location. Linuron catabolic gene clusters that determine the same step were identical on all plasmids, encompassed in differently arranged constellations and characterized by the presence of multiple IS1071 elements. In all plasmids except pEN1, the insertion spot of the catabolic genes in the PromA γ plasmids was the same. Highly similar PromA plasmids carrying the linuron degrading gene cargo at the same insertion spot were previously identified in linuron degrading Variovorax sp. Interestingly, in both Hydrogenophaga populations not every PromA plasmid copy carries catabolic genes. The results indicate that PromA plasmids are important vehicles of linuron catabolic gene dissemination, rather than being cryptic and only important for the mobilization of other plasmids.

Preview

Cite

Citation style:
Could not load citation form.

Access Statistic

Total:
Downloads:
Abtractviews:
Last 12 Month:
Downloads:
Abtractviews:

Rights

License Holder: 2020 Werner, Nour, Bunk, Spröer, Smalla, Springael and Öztürk

Use and reproduction: