Genetic diversity of hull-less barley (Hordeum vulgare L.) landraces in the highlands of central Nepal as revealed by SSRs

Zugehörigkeit
Institute of Crop Science and Plant Breeding I, Justus-Liebig-University Giessen
Pandey, Madhav;
Zugehörigkeit
Institute of Crop Science and Plant Breeding I, Justus-Liebig-University Giessen
Wagner, Carola;
GND
143673491
Zugehörigkeit
Institute of Crop Science and Plant Breeding I, Justus-Liebig-University Giessen
Friedt, Wolfgang;
GND
172295300
Zugehörigkeit
Federal Centre for Breeding Research on Cultivated Plants, Institute of Epidemiology and Resistance Resources
Ordon, Frank

A set of 107 naked barley genotypes from mountains and highlands of Annapurna, Manaslu and Lamtang Himalaya range in central Nepal and 8 selected German and Canadian cultivars were analysed for genetic similarity using 40 SSRs covering the barley genome. Based on this, 258 alleles were detected with an average of 6.45 alleles per SSR. Taking into account only Nepalese barley on average 4.8 alleles were found. The overall diversity index was estimated at 0.53 and within Nepalese landraces at 0.52. The UPGMA clustering based on Dice similarity coefficient clearly separates Nepalese landraces from European and Canadian cultivars. The Nepalese collection is grouped into two distinct clusters and several sub-clusters, which in most cases are in accordance with the collection sites. The SSR markers revealed considerable genetic diversity within the Nepalese naked barley landraces.

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