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A rapid and inexpensive RNA-extraction method for high-throughput virus detection in grapevine

GND
1178348938
Affiliation
Julius Kühn-Institute (JKI), Institute for Plant Protection in Fruit Crops and Viticulture, Germany
Steinmetz, Nadine;
GND
1178348024
Affiliation
Julius Kühn-Institute (JKI), Institute for Plant Protection in Fruit Crops and Viticulture, Germany
Michl, Gertraud;
GND
1059140373
Affiliation
Julius Kühn-Institute (JKI), Institute for Plant Protection in Fruit Crops and Viticulture, Germany
Maixner, Michael;
GND
139264329
Affiliation
Julius Kühn-Institute (JKI), Institute for Plant Protection in Fruit Crops and Viticulture, Germany
Hoffmann, Christoph

The extraction of RNA from grapevine tissue is a crucial step for virus diagnostics via multiplex reverse transcription-polymerase chain reaction (mRT-PCR). Conventional methods are either time-consuming or expensive when convenient extraction kits are used. Here we present an easy, but reliable extraction method that fulfills the requirements of epidemiological research (high sample throughput with maximum accuracy). A further advantage of the protocol beside the low costs is the absence of harmful chemicals like phenol or chloroform and the possibility to manage 'high-throughput' extractions and analyses.

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License Holder: 2020 The Author(s)

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