Article CC BY 4.0
refereed
published

Four types of scrapie in goats differentiated from each other and bovine spongiform encephalopathy by biochemical methods

Affiliation
Wageningen BioVeterinary Research (WBVR), Wageningen University & Research, Houtribweg 39, Lelystad, Netherlands
Langeveld, Jan P M;
Affiliation
Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanita (ISS), Rome, Italy
Pirisinu, Laura;
Affiliation
Wageningen BioVeterinary Research (WBVR), Wageningen University & Research, Houtribweg 39, Lelystad, Netherlands
Jacobs, Jorg G.;
Affiliation
Italian Reference Centre for TSEs, Istituto Zooprofilattico Sperimentale del Piemonte (IZSTO), Turin, Italy
Mazza, Maria;
Affiliation
UMR 1282 ISP, Institut National de la Recherche Agronomique (INRA), University of Tours, Nouzilly, France
Lantier, Isabelle;
Affiliation
Commissariat à l'Énergie Atomique (CEA), Gif-sur-Yvette, France
Simon, Stéphanie;
Affiliation
École Nationale Vétérinaire de Toulouse (ENVT), Toulouse, France
Andréoletti, Olivier;
Affiliation
Research Centre for TSE and Emerging Transmissible Diseases, University of Zaragoza (UNIZAR), Zaragoza, Spain
Acin, Cristina;
Affiliation
Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanita (ISS), Rome, Italy
Esposito, Elena;
GND
101956492X
Affiliation
Friedrich-Loeffler-Institut (FLI), Institute of Novel and Emerging Infectious Diseases, Greifswald, Germany
Fast, Christine;
GND
1019564067
Affiliation
Friedrich-Loeffler-Institut (FLI), Institute of Novel and Emerging Infectious Diseases, Greifswald, Germany
Groschup, Martin H.;
Affiliation
Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh (UEDIN), Easter Bush, Midlothian, United Kingdom
Goldmann, Wilfred; Spiropoulos, John;
Affiliation
School of Pharmacy, Aristotle University of Thessaloniki (AUTh), Thessaloniki, Greece
Sklaviadis, Theodoros;
Affiliation
UMR 1282 ISP, Institut National de la Recherche Agronomique (INRA), University of Tours, Nouzilly, France
Lantier, Frederic;
Affiliation
Hellenic Agricultural Organization DEMETER, Veterinary Research Institute, Thessaloniki, Greece
Ekateriniadou, Loukia;
Affiliation
Veterinary Services (VSC), Ministry of Agriculture, Rural Development and Environment, Cyprus
Papasavva-Stylianou, Penelope;
Affiliation
Wageningen BioVeterinary Research (WBVR), Wageningen University & Research, Houtribweg 39, Lelystad, Netherlands
van Keulen, Lucien J M;
Affiliation
Italian Reference Centre for TSEs, Istituto Zooprofilattico Sperimentale del Piemonte (IZSTO), Turin, Italy
Acutis, Pier-Luigi;
Affiliation
Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanita (ISS), Rome, Italy
Agrimi, Umberto;
Affiliation
Wageningen BioVeterinary Research (WBVR), Wageningen University & Research, Houtribweg 39, Lelystad, Netherlands
Bossers, Alex;
Affiliation
Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanita (ISS), Rome, Italy
Nonno, Romolo

Scrapie in goats has been known since 1942, the archetype of prion diseases in which only prion protein (PrP) in misfolded state (PrPSc) acts as infectious agent with fatal consequence. Emergence of bovine spongiform encephalopathy (BSE) with its zoonotic behaviour and detection in goats enhanced fears that its source was located in small ruminants. However, in goats knowledge on prion strain typing is limited. A European-wide study is presented concerning the biochemical phenotypes of the protease resistant fraction of PrPSc (PrPres) in over thirty brain isolates from transmissible spongiform encephalopathy (TSE) affected goats collected in seven countries. Three different scrapie forms were found: classical scrapie (CS), Nor98/atypical scrapie and one case of CH1641 scrapie. In addition, CS was found in two variants-CS-1 and CS-2 (mainly Italy)-which differed in proteolytic resistance of the PrPres N-terminus. Suitable PrPres markers for discriminating CH1641 from BSE (C-type) appeared to be glycoprofile pattern, presence of two triplets instead of one, and structural (in)stability of its core amino acid region. None of the samples exhibited BSE like features. BSE and these four scrapie types, of which CS-2 is new, can be recognized in goats with combinations of a set of nine biochemical parameters.

Preview

Cite

Citation style:
Could not load citation form.

Access Statistic

Total:
Downloads:
Abtractviews:
Last 12 Month:
Downloads:
Abtractviews:

Rights

Use and reproduction:

Export