Global transcriptomic response of bovine endometrium to blastocyst-stage embryos

Affiliation
School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Ireland
Passaro, C.;
Affiliation
School of Veterinary Science, University of Queensland, Gatton, Australia
Tutt, D.;
Affiliation
School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Ireland
Bagés-Arnal, S.;
Affiliation
School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Ireland
Maicas, C.;
Affiliation
Departamento de Reproducción Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid, Spain
Laguna-Barraza, R.;
Affiliation
Departamento de Reproducción Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid, Spain
Gutierrez-Adán, A.;
Affiliation
School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Ireland
Browne, J.A.;
GND
101960834X
Affiliation
Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut, Neustadt-Mariensee, Germany
Rath, Detlef;
Affiliation
Division of Animal Sciences, University of Missouri, Columbia, United States
Behura, S.K.;
Affiliation
Division of Animal Sciences, University of Missouri, Columbia, United States
Spencer, T.E.;
Affiliation
School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Ireland
Fair, T.;
Affiliation
School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Ireland
Lonergan, P.

The aims of this study were (i) to investigate changes in the global transcriptome of bovine endometrial explants induced by exposure to blastocysts, (ii) to investigate if male and female blastocysts elicit a differential response in the endometrial transcriptome in vitro and (iii) to determine whether bovine endometrium responds to the presence of murine embryos. In Experiment 1, endometrial explants from the same uterus were cultured for 6 h with or without 20 in vitro-produced bovine blastocysts. In Experiment 2, endometrial explants were cultured with male or female bovine blastocysts produced in vitro by IVF either using sex-sorted semen or conventional unsorted semen followed by embryo sexing based on a biopsy. In Experiment 3, endometrial explants were cultured alone or in the presence of bovine blastocysts (n = 25) or murine blastocysts (n = 25). Following culture, explants were snap frozen and stored at −80°C until RNA extraction, qPCR or RNA-Seq. Culture with bovine blastocysts increased endometrial expression of 40 transcripts, all of which were interferon-tau induced. Culture with male or female bovine blastocysts increased transcript abundance of five classic interferon-stimulated genes (MX1, MX2, ISG15, OASY1, RSAD2) in explants; however, there was no difference in abundance of transcripts previously reported to be related to embryonic sex (IFNAR1, IFNAR2, CTGF, ARTN, SLC2A1, SLC2A5). Exposure to murine blastocysts did not elicit any detectable change in transcript abundance. These findings, coupled with our previous data, indicate that very local, interferon-tau-induced changes in endometrial gene expression occur in response to blastocysts; whether such changes play any role in subsequent pregnancy recognition remains to be established.

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