Article CC BY 4.0
refereed
published

Experimental H1N1pdm09 infection in pigs mimics human seasonal influenza infections.

GND
114450855X
Affiliation
Department of Experimental Animal Facilities and Biorisk Management, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany.
Schwaiger, Theresa;
GND
1221288652
Affiliation
Department of Experimental Animal Facilities and Biorisk Management, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany.
Sehl, Julia;
GND
1225574137
Affiliation
Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany.
Karte, Claudia;
GND
1144511291
ORCID
0000-0002-4258-0651
Affiliation
Institute of Immunology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany.
Schäfer, Alexander;
GND
141505168
Affiliation
Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany.
Mettenleiter, Thomas C.;
Affiliation
Institute of Immunology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany.
Hühr, Jane;
GND
123662907
Affiliation
Department of Experimental Animal Facilities and Biorisk Management, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany.
Schröder, Charlotte;
GND
129541958
Affiliation
Institute of Immunology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany.
Köllner, Bernd;
GND
131906879
Affiliation
Department of Experimental Animal Facilities and Biorisk Management, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany.
Ulrich, Reiner Georg;
GND
12231655X
ORCID
0000-0003-4597-248X
Affiliation
Institute of Immunology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany.
Blohm, Ulrike

Pigs are anatomically, genetically and physiologically comparable to humans and represent a natural host for influenza A virus (IAV) infections. Thus, pigs may represent a relevant biomedical model for human IAV infections. We set out to investigate the systemic as well as the local immune response in pigs upon two subsequent intranasal infections with IAV H1N1pdm09. We detected decreasing numbers of peripheral blood lymphocytes after the first infection. The simultaneous increase in the frequencies of proliferating cells correlated with an increase in infiltrating leukocytes in the lung. Enhanced perforin expression in αβ and γδ T cells in the respiratory tract indicated a cytotoxic T cell response restricted to the route of virus entry such as the nose, the lung and the bronchoalveolar lavage. Simultaneously, increasing frequencies of CD8αα expressing αβ T cells were observed rapidly after the first infection, which may have inhibited uncontrolled inflammation in the respiratory tract. Taking together, the results of this study demonstrate that experimental IAV infection in pigs mimics major characteristics of human seasonal IAV infections.

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