Common vole (Microtus arvalis) and bank vole (Myodes glareolus) derived permanent cell lines differ in their susceptibility and replication kinetics of animal and zoonotic viruses.

GND
1144497760
Affiliation
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Novel and Emerging Infectious Diseases, Südufer 10, 17493 Greifswald, Insel Riems, Germany.
Binder, Florian;
GND
1019646985
Affiliation
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Department of Experimental Animal Facilities and Biorisk Management, Bio-Bank, Collection of Cell Lines in Veterinary Virology (CCLV), Südufer 10, 17493, Greifswald, Insel Riems, Germany.
Lenk, Matthias;
GND
1234861828
Affiliation
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Diagnostic Virology, Südufer 10, 17493 Greifswald, Insel Riems, Germany.
Weber, Saskia Carola;
GND
1144507960
Affiliation
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Novel and Emerging Infectious Diseases, Südufer 10, 17493 Greifswald, Insel Riems, Germany.
Stoek, Franziska;
GND
1123164975
Affiliation
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Diagnostic Virology, Südufer 10, 17493 Greifswald, Insel Riems, Germany.
Dill, Veronika;
GND
141848537
Affiliation
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Department of Experimental Animal Facilities and Biorisk Management, Bio-Bank, Collection of Cell Lines in Veterinary Virology (CCLV), Südufer 10, 17493, Greifswald, Insel Riems, Germany.
Reiche, Sven;
GND
1025825543
Affiliation
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Department of Experimental Animal Facilities and Biorisk Management, Bio-Bank, Collection of Cell Lines in Veterinary Virology (CCLV), Südufer 10, 17493, Greifswald, Insel Riems, Germany.
Riebe, Roland;
GND
1026054974
Affiliation
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Diagnostic Virology, Südufer 10, 17493 Greifswald, Insel Riems, Germany.
Wernike, Kerstin;
GND
133501620
Affiliation
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Diagnostic Virology, Südufer 10, 17493 Greifswald, Insel Riems, Germany.
Hoffmann, Donata;
GND
12379420X
Affiliation
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Novel and Emerging Infectious Diseases, Südufer 10, 17493 Greifswald, Insel Riems, Germany; German Center for Infection Research (DZIF), Partner site Hamburg-Lübeck-Borstel-Insel Riems, Germany.
Ziegler, Ute;
Affiliation
Comprehensive Pneumology Center, Research Unit Lung Repair and Regeneration, Helmholtz Zentrum München - German Research Center for Environmental Health (GmbH), Marchioninistrasse 25, D-81377 Munich, Germany; University Hospital Grosshadern, Ludwig-Maximilians-University, Munich, Germany.
Adler, Heiko;
Affiliation
Bundeswehr Institute of Microbiology, Department Virology and Rickettsiology, Neuherbergstr. 11, 80937 Munich, Germany.
Essbauer, Sandra;
GND
1019565543
Affiliation
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Novel and Emerging Infectious Diseases, Südufer 10, 17493 Greifswald, Insel Riems, Germany; German Center for Infection Research (DZIF), Partner site Hamburg-Lübeck-Borstel-Insel Riems, Germany. Electronic address: Rainer.Ulrich@fli.de.
Ulrich, Rainer

Pathogenesis and reservoir host adaptation of animal and zoonotic viruses are poorly understood due to missing adequate cell culture and animal models. The bank vole (Myodes glareolus) and common vole (Microtus arvalis) serve as hosts for a variety of zoonotic pathogens. For a better understanding of virus association to a putative animal host, we generated two novel cell lines from bank voles of different evolutionary lineages and two common vole cell lines and assayed their susceptibility, replication and cytopathogenic effect (CPE) formation for rodent-borne, suspected to be rodent-associated or viruses with no obvious rodent association. Already established bank vole cell line BVK168, used as control, was susceptible to almost all viruses tested and efficiently produced infectious virus for almost all of them. The Puumala orthohantavirus strain Vranica/Hällnäs showed efficient replication in a new bank vole kidney cell line, but not in the other four bank and common vole cell lines. Tula orthohantavirus replicated in the kidney cell line of common voles, but was hampered in its replication in the other cell lines. Several zoonotic viruses, such as Cowpox virus, Vaccinia virus, Rift Valley fever virus, and Encephalomyocarditis virus 1 replicated in all cell lines with CPE formation. West Nile virus, Usutu virus, Sindbis virus and Tick-borne encephalitis virus replicated only in a part of the cell lines, perhaps indicating cell line specific factors involved in replication. Rodent specific viruses differed in their replication potential: Murine gammaherpesvirus-68 replicated in the four tested vole cell lines, whereas murine norovirus failed to infect almost all cell lines. Schmallenberg virus and Foot and mouth disease virus replicated in some of the cell lines, although these viruses have never been associated to rodents. In conclusion, these newly developed cell lines may represent useful tools to study virus-cell interactions and to identify and characterize host cell factors involved in replication of rodent associated viruses.

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