Chromosomal assignment of radish linkage groups using a complete set of disomic rapeseed-radish chromosome addition lines

Radish, Raphanus sativus L., is a vegetable with high economic importance, especially in Asia. A genetic map with 322 markers and a total length of 673 cM was anchored to chromosomes using a complete set of disomic rapeseed-radish chromosome addition lines. Eighteen SSRs and one CAPS marker, at least two for each linkage group, were assigned to the individual radish chromosomes. Three of the radish chromosomes (C, D and H) were characterized cytogenetically by fluorescence in situ hybridization (FISH). The anchored linkage map can be used for QTL analysis of morphological characters and other traits.