Artikel CC BY 4.0
referiert
Veröffentlicht

High resolution mapping of RphMBR1012 conferring resistance to Puccinia hordei in barley (Hordeum vulgare L.)

GND
1171448643
Zugehörigkeit
Julius Kuehn Institute (JKI), Institute for Resistance Research and Stress Tolerance, Quedlinburg, Germany; Department of Phytopathology and Plant Protection, Institute of Agricultural and Nutrition Sciences, Faculty of Natural Sciences III, Martin Luther University of Halle-Wittenberg, Halle, Germany
Fazlikhani, Leila;
GND
1013858662
Zugehörigkeit
Julius Kühn-Institut (JKI), Federal Research Centre of Cultivated Plants, Institute for Biosafety in Plant Biotechnology, Quedlinburg , Deutschland
Keilwagen, Jens;
GND
1059141396
Zugehörigkeit
Julius Kuehn Institute (JKI), Institute for Resistance Research and Stress Tolerance, Quedlinburg, Germany
Kopahnke, Doris;
Zugehörigkeit
Department of Phytopathology and Plant Protection, Institute of Agricultural and Nutrition Sciences, Faculty of Natural Sciences III, Martin Luther University of Halle-Wittenberg, Halle, Germany
Deising, Holger;
GND
172295300
Zugehörigkeit
Julius Kuehn Institute (JKI), Institute for Resistance Research and Stress Tolerance, Quedlinburg, Germany
Ordon, Frank;
GND
1059141701
Zugehörigkeit
Julius Kuehn Institute (JKI), Institute for Resistance Research and Stress Tolerance, Quedlinburg, Germany
Perovic, Dragan

Isolation of disease resistance genes in barley was hampered by the large genome size, but has become easy due to the availability of the reference genome sequence. During the last years, many genomic resources, e.g., the Illumina 9K iSelect, the 50K Infinium arrays, the Barley Genome Zipper, POPSEQ, and genotyping by sequencing (GBS), were developed that enable enhanced gene isolation in combination with the barley genome sequence. In the present study, we developed a fine map of the barley leaf rust resistance gene RphMBR1012. 537 segmental homozygous recombinant inbred lines (RILs) derived from 4775 F2-plants were used to construct a high-resolution mapping population (HRMP). The Barley Genome Zipper, the 9K iSelect chip, the 50K Infinium chip and GBS were used to develop 56 molecular markers located in the target interval of 8 cM. This interval was narrowed down to about 0.07 cM corresponding to 0.44 Mb of the barley reference genome. Eleven low-confidence and 18 high-confidence genes were identified in this interval. Five of these are putative disease resistance genes and were subjected to allele-specific sequencing. In addition, comparison of the genetic map and the reference genome revealed an inversion of 1.34 Mb located distally to the resistance locus. In conclusion, the barley reference sequence and the respective gene annotation delivered detailed information about the physical size of the target interval, the genes located in the target interval and facilitated the efficient development of molecular markers for marker-assisted selection for RphMBR1012:

Vorschau

Zitieren

Zitierform:
Zitierform konnte nicht geladen werden.

Zugriffsstatistik

Gesamt:
Volltextzugriffe:
Metadatenansicht:
12 Monate:
Volltextzugriffe:
Metadatenansicht:

Rechte

Rechteinhaber: 2019 Fazlikhani, Keilwagen, Kopahnke, Deising, Ordon and Perovic.

Nutzung und Vervielfältigung: