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Towards map-based cloning of FB_Mfu10: identification of a receptor-like kinase candidate gene underlying the Malus fusca fire blight resistance locus on linkage group 10

GND
1211645738
Affiliation
Julius Kühn-Institute (JKI), Institute for Breeding Research on Fruit Crops, Germany
Emeriewen, Ofere F.;
GND
1059141744
Affiliation
Julius Kühn-Institute (JKI), Institute for Resistance Research and Stress Tolerance, Germany
Richter, Klaus;
Affiliation
Research and Innovation Centre, Fondazione Edmund Mach (FEM), San Michele all ‘Adige, Italy
Piazza, Stefano;
Affiliation
Research and Innovation Centre, Fondazione Edmund Mach (FEM), San Michele all ‘Adige, Italy
Micheletti, Diego;
Affiliation
Swiss Federal Institute of Technology, Molecular Plant Breeding, Zurich, Switzerland
Broggini, Giovanni A. I.;
GND
1172920559
Affiliation
Julius Kühn-Institute (JKI), Institute for Biosafety in Plant Biotechnology, Germany
Berner, Thomas;
GND
1013858662
Affiliation
Julius Kühn-Institute (JKI), Institute for Biosafety in Plant Biotechnology, Germany
Keilwagen, Jens;
GND
1059103400
Affiliation
Julius Kühn-Institute (JKI), Institute for Breeding Research on Fruit Crops, Germany
Hanke, Magda-Viola;
Affiliation
Research and Innovation Centre, Fondazione Edmund Mach (FEM), San Michele all ‘Adige, Italy
Malnoy, Michael;
GND
172861896
Affiliation
Julius Kühn-Institute (JKI), Institute for Breeding Research on Fruit Crops, Germany
Peil, Andreas

Breeding for resistance against the destructive fire blight disease of apples is the most sustainable strategy to control the menace of this disease, and has become increasingly important in European apple breeding programs. Since most cultivars are susceptible, wild accessions have been explored for resistance with quantitative trait loci detected in a few wild species. Fire blight resistance of Malus fusca was described following phenotypic evaluations with a C-type strain of Erwinia amylovora, Ea222_JKI, and the detection of a major QTL on chromosome 10 (Mfu10) of this crabapple. The stability of the resistance of M. fusca and Mfu10 has been evaluated using two other strains, the highly aggressive Canadian S-type strain—Ea3049, and the avrRpt2EA mutant—ZYRKD3-1, both of which overcome the resistance of Malus ×robusta 5, a wild species accession with an already described fire blight resistance gene. To pave the way for positional cloning of the underlying fire blight resistance gene of M. fusca, we have fine mapped the QTL region on linkage group 10 using 1888 individuals and 23 newly developed molecular markers, thus delimiting the interval of interest to 0.33 cM between markers FR39G5T7xT7y/FR24N24RP and FRMf7358424/FR46H22. Tightly linked SSR markers are suitable for marker-assisted selection in breeding programs. Furthermore, a bacterial artificial chromosome (BAC) clone spanning FB_Mfu10 region was isolated and sequenced. One putative fire blight resistance candidate gene of M. fusca was predicted on the sequence of BAC 46H22 within the resistance region that encodes B-lectin and serine/threonine kinase domains.

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