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Improved genetic resolution for linkage mapping of resistance to potato wart in monoparental dihaploids with potential diagnostic value in tetraploid potato varieties

Affiliation
Institute of Plant Genetics, Department of Molecular Plant Breeding, Leibniz University Hannover, Hannover, Germany ; DHD-Consulting GmbH, Hildesheim, Germany
Bartkiewicz, Annette;
GND
1166702766
Affiliation
Institute of Plant Genetics, Department of Molecular Plant Breeding, Leibniz University Hannover, Hannover, Germany ; Julius Kuehn Institute (JKI), Institute of Plant Protection in Field Crops and Grassland, Kleinmachnow, Germany
Chilla, Friederike;
Affiliation
Institute of Plant Genetics, Department of Molecular Plant Breeding, Leibniz University Hannover, Hannover, Germany ; Westhoff, Südlohn-Oeding, Germany
Terefe-Ayana, Diro;
Affiliation
SaKa Pflanzenzucht GmbH & Co. KG, Windeby, Germany
Lübeck, Jens;
Affiliation
SaKa Pflanzenzucht GmbH & Co. KG, Windeby, Germany
Strahwald, Josef;
Affiliation
Böhm-Nordkartoffel Agrarproduktion GmbH & Co. OHG, Ebstorf, Germany
Tacke, Eckhard;
Affiliation
Böhm-Nordkartoffel Agrarproduktion GmbH & Co. OHG, Ebstorf, Germany
Hofferbert, Hans-Reinhard;
GND
1058937626
Affiliation
Julius Kuehn Institute (JKI), Institute of Plant Protection in Field Crops and Grassland, Kleinmachnow, Germany
Flath, Kerstin;
Affiliation
Institute of Plant Genetics, Department of Molecular Plant Breeding, Leibniz University Hannover, Hannover, Germany
Linde, Marcus;
Affiliation
Institute of Plant Genetics, Department of Molecular Plant Breeding, Leibniz University Hannover, Hannover, Germany
Thomas Debener

We analyzed a segregating monoparental dihaploid potato population comprising 215 genotypes derived from a tetraploid variety that is highly resistant to Synchytrium endobioticum pathotypes 18 and 6. The clear bimodal segregation for both pathotypes indicated that a major dominant resistance factor in a simplex allele configuration was present in the tetraploid donor genotype. Compared to that in previous analyses of the same tetraploid donor in conventional crosses with susceptible tetraploid genotypes, a segregation pattern with a reduced genetic complexity of resistance in dihaploids was observed here. Using the 12.8 k SolCAP SNP array, we mapped a resistance locus to the Xla-TNL region containing also Sen1 on potato chromosome 11. The improved mapping resolution provided by the monoparental dihaploids allowed for the localization of the genes responsible for the resistance to both pathotypes in an interval spanning less than 800 kbp on the reference genome. Furthermore, we identified eight molecular markers segregating without recombination to pathotype 18 and pathotype 6 resistance. Also, two developed markers display improved diagnostic properties in an independent panel of tetraploid varieties. Overall, our data provide the highest resolution mapping of wart resistance genes at the Xla-TNL locus thus far.

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