Article All rights reserved
refereed
published

Nanovirus DNA-N encodes a protein mandatory for aphid transmission

ORCID
0000-0003-2547-8935
Affiliation
Institut des Sciences du Végétal, CNRS, Gif sur Yvette, France
Grigoras, Ioana;
GND
1145734251
Affiliation
Julius Kuehn-Institute, Institute for Epidemiology and Pathogen Diagnostics, Brunswick, Germany
Vetten, Heinrich-Josef;
Affiliation
Institute for Molecular Biotechnology (Biology VII), RWTH Aachen University, Aachen, Germany
Commandeur, Ulrich;
GND
1058967991
Affiliation
Julius Kuehn-Institute, Institute for Epidemiology and Pathogen Diagnostics, Brunswick, Germany
Ziebell, Heiko;
Affiliation
Institut des Sciences du Végétal, CNRS, Gif sur Yvette, France ; Institute for Integrative Biology of the Cell, CNRS, Université Paris-Sud, Gif sur Yvette, France
Gronenborn, Bruno;
Affiliation
Institut des Sciences du Végétal, CNRS, Gif sur Yvette, France ; Institute for Integrative Biology of the Cell, CNRS, Université Paris-Sud, Gif sur Yvette, France
Timchenko, Tatiana

Nanoviruses possess a multipartite single-stranded DNA genome and are naturally transmitted to plants by various aphid species in a circulative non-propagative manner. Using the cloned genomic DNAs of faba bean necrotic stunt virus (FBNSV) for reconstituting nanovirus infections we analyzed the necessity of different virus components for infection and transmission by aphids. We found that in the absence of DNA-U1 and DNA-U2 symptom severity decreased, and in the absence of DNA-U1 the transmission efficiency decreased. Most significantly, we demonstrated that the protein encoded by DNA-N (NSP) is mandatory for aphid transmission. Moreover, we showed that the NSP of FBNSV could substitute for that of a distantly related nanovirus, pea necrotic yellow dwarf virus. Altering the FBNSV NSP by adding 13 amino acids to its carboxy-terminus resulted in an infectious but non-transmissible virus. We demonstrate that the NSP acts as a nanovirus transmission factor, the existence of which had been hypothesized earlier.

Files

Cite

Citation style:
Could not load citation form.

Access Statistic

Total:
Downloads:
Abtractviews:
Last 12 Month:
Downloads:
Abtractviews:

Rights

License Holder: 2018 Elsevier Inc.

Use and reproduction:
All rights reserved

Export