Flow cytometry as a new complementary tool to study Coxiella burnetii in cell cultures

Rebbig, Annica; Matthiesen, Svea; Lührmann, Anja; Knittler, Michael R. GND

Flow cytometry enables the analysis of cells by labeling them with fluorescent probes. We describe a novel flow cytometric approach permitting reliable analysis of Coxiella (C.) burnetii-infected cells. The method quantifies infection-forming units (IFUs) in a dose-dependent manner and allows for the specific detection of infection/replication-competent coxiella in cell cultures.

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Rebbig, Annica / Matthiesen, Svea / Lührmann, Anja / et al: Flow cytometry as a new complementary tool to study Coxiella burnetii in cell cultures. 2018.

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